Product details for  CD90.2 Mouse (Murine) antibody
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CD90.2 Mouse (Murine) antibody

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Image for CD90.2 Mouse (Murine) antibody
Antigen: CD90.2
Antibody Type: Monoclonal
Application: Flow Cytometry (FACS), Depletion, Costimulation, Immunohistochemistry (Frozen Sections) (IHC (fro))
Conjugate: LEAF
Reactivity: Mouse (Murine)
Host: Rat
Quantity: 500 µg (Product variants)
Price: 289,00 €   (Plus shipping costs and VAT)
Order number: ABIN156086
Availability: Ships within 7 to 10 days
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Additional Information:  CD90.2 Mouse (Murine) antibody

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Antigen CD90.2
Immunogen Mouse thymus or spleen
Reactivity Mouse (Murine)
Antibody Type Monoclonal
Format Sterile Low-Endotoxin, Azide-Free formulation (LEAF)
Isotype Rat IgG2b, kappa »Matching secondary antibodies
Description CD90.2 is a 25-35 kD immunoglobulin superfamily member also known as Thy-1.2. It is expressed on hematopoietic stem cells and neurons, all thymocytes, and peripheral T cells in Thy1.2 bearing mouse strains (Balb/c, CBA/J, C3H/He, C57BL/-, DBA, NZB/-). CD90.2 is a glycosylphosphatidylinositol (GPI)-anchored membrane glycoprotein involved in signal transduction. CD90.2 is involved in costimulation of lymphocyte proliferation and induction of hematopoietic stem cells differentiation. CD90.2 has been shown to interact with CD45. The 30-H12 antibody has been reported to induce Ca flux in thymocytes and, in combination with antibody against the CD3/TCR complex, promote thymocyte apoptosis and inhibit CD3-mediated proliferative responses of mature T lymphocytes. Distribution: Hematopoietic stem cells and neurons, all thymocytes, peripheral T cells of the Thy-1.2 bearing mice Function: Lymphocyte costimulation, proliferation and differentiation of hematopoietic stem cells Synonyms: Thy-1.2 Structure: Ig superfamily, 25-35 kD
Clone 30-H12
Host Rat

Application Details:  CD90.2 Mouse (Murine) antibody

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Application Flow Cytometry (FACS), Depletion, Costimulation, Immunohistochemistry (Frozen Sections) (IHC (fro))
Application Notes Additional reported applications (for the relevant formats) include: in vivo and in vitro depletion, costimulation of CD3/TCR-mediated signal transduction, and immunohistochemical staining of acetone-fixed frozen sections. The 30-H12 antibody does not react with Thy-1.1 alloantigen of the AKR/J and PL strains. To reduce non-specific binding to cells bearing Fc-receptors, pre-incubation of cells with anti-mouse CD16/CD32, clone 93 (ABIN155518/ABIN155519), is recommended prior to immunofluorescent staining. The LEAF(TM) purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm sterile-filtered) is recommended for functional assays (ABIN156086). Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is < 0.25 µg per 10 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Conjugate LEAF
Purification The antibody was purified by affinity chromatography.
Buffer Phosphate-buffered solution, pH 7.2, containing no preservative. 0.2 µm filter sterilized. Endotoxin level is < 0.1 EU/µg of the protein (< 0.01 ng/µg of the protein) as determined by the LAL test.
Storage The antibody solution should be stored undiluted at 4 °C. This LEAF(TM) solution contains no preservative, handle under aseptic conditions.
Research Area CD Antigens
Restrictions For Research Use only

Publications:  CD90.2 Mouse (Murine) antibody

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Publications Craig, Kay, Cutler et al.:
"Expression of Thy-1 on human hematopoietic progenitor cells."
In: The Journal of experimental medicine , Vol. 177, Issue 5, pp. 1331-42, 1993/01 (PubMed)

Mayani, Lansdorp:
"Thy-1 expression is linked to functional properties of primitive hematopoietic progenitor cells from human umbilical cord blood."
In: Blood , Vol. 83, Issue 9, pp. 2410-7, 1994/01 (PubMed)

Drobyski, Majewski:
"Treatment of donor mice with an alpha beta T-cell receptor monoclonal antibody induces prolonged T-cell nonresponsiveness and effectively prevents lethal graft-versus-host disease in murine recipients of major histocompatibility complex (MHC)-matched and "
In: Blood , Vol. 87, Issue 12, pp. 5355-69, 1996/01 (PubMed)

Dyer, Czapiga, Foster et al.:
"Eosinophils from lineage-ablated Delta dblGATA bone marrow progenitors: the dblGATA enhancer in the promoter of GATA-1 is not essential for differentiation ex vivo."
In: Journal of immunology (Baltimore, Md. : 1950) , Vol. 179, Issue 3, pp. 1693-9, 2007/01 (PubMed)

Hardy, Niv, Fadaeev et al.:
"BAT mAb induces lymphopoiesis in nude mice."
In: International immunology , Vol. 17, Issue 5, pp. 615-9, 2005/01 (PubMed)

Ledbetter, Rouse, Micklem et al.:
"T cell subsets defined by expression of Lyt-1,2,3 and Thy-1 antigens. Two-parameter immunofluorescence and cytotoxicity analysis with monoclonal antibodies modifies current views."
In: The Journal of experimental medicine , Vol. 152, Issue 2, pp. 280-95, 1980/01 (PubMed)

Nakashima, Zhang, Rahman et al.:
"Evidence of synergy between Thy-1 and CD3/TCR complex in signal delivery to murine thymocytes for cell death."
In: Journal of immunology (Baltimore, Md. : 1950) , Vol. 147, Issue 4, pp. 1153-62, 1991/01 (PubMed)

Nakashima, Pu, Hamaguchi et al.:
"Pathway of signal delivery to murine thymocytes triggered by co-crosslinking CD3 and Thy-1 for cellular DNA fragmentation and growth inhibition."
In: Journal of immunology (Baltimore, Md. : 1950) , Vol. 151, Issue 7, pp. 3511-20, 1993/01 (PubMed)

Seaman, Wofsy, Greenspan et al.:
"Treatment of autoimmune MRL/Ipr mice with monoclonal antibody to Thy-1.2: a single injection has sustained effects on lymphoproliferation and renal disease."
In: Journal of immunology (Baltimore, Md. : 1950) , Vol. 130, Issue 4, pp. 1713-8, 1983/01 (PubMed)

Images:  CD90.2 Mouse (Murine) antibody

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C57BL/6 thymocytes stained with 30-H12 FITC

Image for CD90.2 Mouse (Murine) antibody (1)

External Information:  CD90.2 Mouse (Murine) antibody

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Google Scholar Find more references for clone 30-H12 on Google Scholar™
EMBL Harvester Search human, mouse or rat genome for antigen CD90.2 (Bioinformatic Harvester (EMBL))