CEACAM5 (Human) antibody

Overview


	Antigen:	CEACAM5 (CEA, CD66e)
	Antibody Type:	Monoclonal
	Application:	Flow Cytometry (FACS), Cell-ELISA, Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
	Reactivity:	Human
	Host:	Mouse
	Quantity:	100 ug (1mg/ml)
	Price:	250,00 € (Plus shipping costs and VAT)
	Order number:	ABIN108727
	Availability:	1 to 2 days until shipment

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Additional Information: CEACAM5 (Human) antibody

Antigen	CEACAM5 (CEA, CD66e)
Immunogen	TET2 was generated by immunisation of BALB/c mice with CEA partially purified from a perchloric acid extract from liver metastases of colonic tumors (3). The antibody was purified by protein G affinity chromatography from cell culture supernatants and is
Reactivity	Human
Antibody Type	Monoclonal
Format	Purified
Isotype	IgG1 »Matching secondary antibodies
Description	CEACAM5 (CEA-related cell adhesion molecule 5, CEA) belongs to the carcinoembryonic antigen (CEA) gene family (5). It encodes a glycosyl phosphatidylinositol (GPI)- linked glycoprotein with a Mr of 180,000-200,000 which is most strongly expressed on epithelial cells of the fetal and adult colon and to a minor extent on epithelial cells of the stomach and sweat glands, squamous epithelial cell of the tongue, esophagus and cervix. CEACAM5 is used as a tumor marker for early detection of recurrent disease due its expression in adenocarcinomas of the colon, lung, breast, stomach and pancreas and in mucinous ovarian carcinomas (5). Like all members of the CEACAM family, it consists of a single N domain, with structural homology to the immunoglobulin variable domains, followed by six immunoglobulin constant-like A (A1, A2, A3) and B domains (B1, B2, B3).
Clone	26-3-13
Host	Mouse
Specificity	TET2 is a broadly reactive antibody which reacts with most of the CEACAM molecules characterized to date (except CEACAM3, CEACAM4 and CEACAM7)4. TET2 can be used to detect CEACAM1 (BGP/CD66a), CEACAM5 (CEA/CD66e), CEACAM6 (NCA/CD66c) and CEACAM8 (CGM6/CD6

Application Details: CEACAM5 (Human) antibody

Application	Flow Cytometry (FACS), Cell-ELISA, Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
Application Notes	TET2 has been tested for the use in flow cytometry (1.2 ug/10^6 cells), cell-ELISA (1:200), ELISA (1:200 - 1:400), Western blots (4ug/ml) and immunohistology on cryosections (1-2 ug/10^6 cells).
Concentration	1mg/ml
Purification	Protein G
Buffer	Phosphate buffered saline, pH 7.2
Storage	Short term: 2°C - 8°C. Long term: -20°C (avoid repeated freezing and thawing)
Research Area	Receptors, Cancer, Receptors, Cell/Tissue Markers, Cancer
Restrictions	For research use only. Not for diagnostic or therapeutic use. Caution hazardous ingredient: Sodium azide, avoid exposure to skin and eyes, ingestion, and contact with heat, acids, and metals or metal ions. Wash exposed skin with soap and water. Flush eye

Publications: CEACAM5 (Human) antibody

Publications

Baczy?ska, Wietrzyk, Madej et al.:
"The tumorigenic potential of human CX-1 colon adenocarcinoma cells depends on carcinoembryonic antigen (CEACAM5) expression."
In: Cellular & molecular biology letters , Vol. 8, Issue 2, pp. 471-86, 2003/01 (PubMed)

Zimmermann W (2002):
"Carcinoembryonic antigen"
In: In Wiley Encyclopedia of Molecular Medicine , pp. 459-462

Schoelzel, Zimmermann, Schwarzkopf et al.:
"Carcinoembryonic antigen family members CEACAM6 and CEACAM7 are differentially expressed in normal tissues and oppositely deregulated in hyperplastic colorectal polyps and early adenomas."
In: The American journal of pathology , Vol. 156, Issue 2, pp. 595-605, 2000/01 (PubMed)

Hammarström S:
"The carcinoembryonic antigen (CEA) family: structures, suggested functions and expression in normal and malignant tissues."
In: Semin. Cancer Biol. , Vol. 9, pp. 67-81

Grunert F, Stocks SC, Nagel G et al.:
"CD66 family Workshop: Binding of myeloid blind panel antibodies and CD66 Subsection antibodies to HeLa transfectants expressing individual CD66 molecules."
In: In Leukocyte Typing VI: White cell Differentiation Antigens T. Kishimoto et al., eds., Garland Publishing Inc., New York and London , pp. 1012-1025

Thompson, Moessinger, Reichardt et al.:
"A polymerase-chain-reaction assay for the specific identification of transcripts encoded by individual carcinoembryonic antigen (CEA)-gene-family members."
In: International journal of cancer. Journal international du cancer , Vol. 55, Issue 2, pp. 311-9, 1993/01 (PubMed)

Strobel, Strobel, Bross et al.:
"Effects of human bone marrow stroma on the growth of human tumor cells."
In: Cancer research , Vol. 49, Issue 4, pp. 1001-7, 1989/01 (PubMed)

Grunert, AbuHarfeil, Schwarz et al.:
"Two CEA and three NCA species, although distinguishable by monoclonal antibodies, have nearly identical peptide patterns."
In: International journal of cancer. Journal international du cancer , Vol. 36, Issue 3, pp. 357-62, 1985/01 (PubMed)

Krop-Watorek, Laskowska, Salwa et al.:
"CEA-related proteins on human urothelial cell lines of different transformation grades."
In: Cancer letters , Vol. 139, Issue 1, pp. 15-22, 1999/01 (PubMed)

Images: CEACAM5 (Human) antibody

Specificity testing of 26/3/13. Members of the CEA family were expressed on BOSC cells after transient transfection with expression vectors containing either the cDNA of CEACAM1, 5, 6, 7 or 8. Recognition of CEACAM3, 4 and of a recombinant transmembrane-

Image for CEACAM5 (Human) antibody (1)

FACS analysis of BOSC23 cells using 26/3/13. BOSC23 cells were transiently transfected with an expres-sion vector encoding either CEACAM5 (red curve) or an irrelevant protein (control transfectant). Binding of 26/3/13 was detected witha PE conjugated sec

Image for CEACAM5 (Human) antibody (2)

External Information: CEACAM5 (Human) antibody

Google Scholar	Find more references for clone 26-3-13 on Google Scholar™
EMBL Harvester	Search human, mouse or rat genome for antigen CEACAM5 (CEA, CD66e) (Bioinformatic Harvester (EMBL))