CLPB (Human) antibody

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抗原	CLPB
基因ID	81570
免疫原	Peptide with sequence C-DKDSKTRRLDIRAP, from the C Terminus of the protein sequence according to NP_110440.1
目标物种	Human
抗体类型	多克隆抗体
状态	Aff - Purified
描述	Official Gene Symbol: CLPB Accession Number(s): NP_110440.1. . Principal Names: CLPB, ClpB caseinolytic peptidase B homolog (E. coli), FLJ13152, HSP78, SKD3, suppressor of potassium transport defect 3.
抗体来源	Goat
特异性	Expected reactivity from sequence similarity: Human

应用备注	Peptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Preliminary experiments gave bands at approx 50kDa and 48kDa in lysates of HeLa and HepG2 after 0.1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 78.7kDa according to NP_110440.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
纯化	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
缓冲液	0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
贮藏	Aliquot and store at -20°C. Minimize freezing and thawing.
限制	For Research Use Only