CLPB (Human) antibody

Overview

Antigen	CLPB
Gene ID	81570
Immunogen	Peptide with sequence C-DKDSKTRRLDIRAP, from the C Terminus of the protein sequence according to NP_110440.1
Reactivity	Human
Antibody Type	Polyclonal
Format	Aff - Purified
Description	Official Gene Symbol: CLPB Accession Number(s): NP_110440.1. . Principal Names: CLPB, ClpB caseinolytic peptidase B homolog (E. coli), FLJ13152, HSP78, SKD3, suppressor of potassium transport defect 3.
Host	Goat
Specificity	Expected reactivity from sequence similarity: Human

Application Notes	Peptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Preliminary experiments gave bands at approx 50kDa and 48kDa in lysates of HeLa and HepG2 after 0.1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 78.7kDa according to NP_110440.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Format	Aff - Purified
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Buffer	0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Storage	Aliquot and store at -20°C. Minimize freezing and thawing.
Restrictions	For Research Use Only

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