Cullin 1 (C-term.) (Human) antibody

Overview


	Antigen:	Cullin 1 (C-term.)
	Antibody Type:	Polyclonal
	Application:	Enzyme Immunoassay (EIA), Immunoprecipitation (IP), Western Blotting (WB)
	Reactivity:	Human, Mouse (Murine)
	Host:	Rabbit
	Quantity:	0.1 ml (85.0 mg/ml (by Refractometry))
	Price:	462,23 $ (Plus shipping costs and VAT, Invoice in EUR)
	Order number:	ABIN117947
	Availability:	Ships in 3 to 10 days

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Additional Information: Cullin 1 (C-term.) (Human) antibody

Antigen	Cullin 1 (C-term.)
Immunogen	Synthetic peptide corresponding to amino acids 742-752 of Human Cul1 (C-terminus) coupled to KLH.
Reactivity	Human, Mouse (Murine)
Antibody Type	Polyclonal
Format	Serum
Host	Rabbit

Application Details: Cullin 1 (C-term.) (Human) antibody

Application	Enzyme Immunoassay (EIA), Immunoprecipitation (IP), Western Blotting (WB)
Application Notes	This antibody reacts with human Cul1 by Western blot (1/500-1/1,000, An 89.6 kDa band corresponding to human Cul1 is detected, Most cell lines expressing Cul1 can be used as a positive control) and immunoprecipitation (The antibody immunoprecipitates in vitro translated product and protein from cell lysates (using HeLa or NIH-3T3). Do not IP in the presence of NP-40, but rather use 0.1% SDS). ELISA (1/2,000-1/10,000). Other applications not tested. Optimal dilutions of this antibody are dependent on conditions and should be determined by the user. Purity and Specificity: This product is monospecific antiserum processed by delipidation and defibrination followed by sterile filtration. This product reacts with human Cullin 1. Cross reactivity is expected against mouse Cul1 based on sequence homology. Cullins assemble a potentially large number of ubiquitin ligases by binding to the RING protein ROC1 to catalyse polyubiquitination, as well as binding to various specificity factors to recruit substrates. Cullin 1 is an essential component of the SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complex, which mediates the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription. In the SCF complex, cul1 serves as a rigid scaffold that organizes the SKP1-F-box protein and RBX1 subunits. Cul1 may also contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. Cul1 is part of the SCF complex consisting of CUL1, RBX1, SKP1 and SKP2, where it interacts directly with SKP1, SKP2 and RBX1. Cul1 also interacts with RNF7 and is part of a complex with TIP120A/CAND1 and RBX1. The unneddylated form interacts with TIP120A/CAND1 and the interaction negatively regulates the association with SKP1 in the SCF complex.
Concentration	85.0 mg/ml (by Refractometry)
Buffer	This purified antibody is supplied as sterile filtered, liquid serum containing 0.01% (w/v) Sodium Azide as preservative.
Storage	Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. Conjugation pathways for ubiquitin and ubiquitin-like modifiers (UBLs). Most modifiers mature by proteolytic processing from inactive precursors (a, amino acid). Arrowheads point to the cleavage sites. Ubiquitin is expressed either as polyubiquitin or as a fusion with ribosomal proteins. Conjugation requires activating (E1) and conjugating (E2) enzymes that form thiolesters (S) with the modifiers. Modification of cullins by RUB involves SCF(SKP1/cullin-1/F-box protein) /CBC(cullin-2/elongin B/elonginC) -like E3 enzymes that are also involved in ubiquitination. In contrast to ubiquitin, the UBLs do not seem to form multi-UBL chains. UCRP(ISG15) resembles two ubiquitin moieties linked head-to-tail. Whether HUB1 functions as a modifier is currently unclear. APG12 and URM1 are distinct from the other modifiers because they are unrelated in sequence to ubiquitin. Data contributed by S.Jentsch, see references below. Protein Sequence: Human Cullin 1 (776 aa 89.6 kDa) 1 msstrsqnph glkqigldqi wddlragiqq vytrqsmaks rymelythvy nyctsvhqsn 61 qargagvpps kskkgqtpgg aqfvglelyk rlkeflknyl tnllkdgedl mdesvlkfyt 121 qqwedyrfss kvlngicayl nrhwvrrecd egrkgiyeiy slalvtwrdc lfrplnkqvt 181 navlklieke rngetintrl isgvvqsyve lglneddafa kgptltvyke sfesqfladt 241 erfytreste flqqnpvtey mkkaearlle eqrrvqvylh estqdelark ceqvliekhl 301 eifhtefqnl ldadknedlg rmynlvsriq dglgelkkll ethihnqgla aiekcgeaal 361 ndpkmyvqtv ldvhkkynal vmsafnndag fvaaldkacg rfinnnavtk maqssskspe 421 llarycdsll kkssknpeea eledtlnqvm vvfkyiedkd vfqkfyakml akrlvhqnsa 481 sddaeasmis klkqacgfey tsklqrmfqd igvskdlneq fkkhltnsep ldldfsiqvl 541 ssgswpfqqs ctfalpsele rsyqrftafy asrhsgrklt wlyqlskgel vtncfknryt 601 lqastfqmai llqynteday tvqqltdstq ikmdilaqvl qillkskllv ledenanvde 661 velkpdtlik lylgyknkkl rvninvpmkt eqkqeqetth knieedrkll iqaaivrimk 721 mrkvlkhqql lgevltqlss rfkprvpvik kcidilieke ylervdgekd tysyla
Research Area	Apoptosis/Necrosis, Proteasome
Restrictions	Research only

Images: Cullin 1 (C-term.) (Human) antibody

Conjugation pathways for ubiquitin and.,.ubiquitin-like modifiers (UBLs). Most modifiers mature by proteolytic processing from inactive precursors (a, amino acid). Arrowheads point to the cleavage sites. Ubiquitin is expressed either as polyubiquitin or as a fusion with ribosomal proteins. Conjugation requires activating (E1) and conjugating (E2) enzymes that form thiolesters (S) with the modifiers. Modification of cullins by RUB involves SCF(SKP1/cullin-1/F-box protein) /CBC(cullin-2/elongin B/elonginC) -like E3 enzymes that are also involved in ubiquitination. In contrast to ubiquitin, the UBLs do not seem to form multi-UBL chains. UCRP(ISG15) resembles two ubiquitin moieties linked head-to-tail. Whether HUB1 functions as a modifier is currently unclear. APG12 and URM1 are distinct from the other modifiers because they are unrelated in sequence to ubiquitin. Data contributed by S.Jentsch, see references below.

Image for Cullin 1 (C-term.) (Human) antibody (1)

External Information: Cullin 1 (C-term.) (Human) antibody

Google Scholar	Find more references for antigen Cullin 1 (C-term.) on Google Scholar™
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