Cullin 2 (C-term.) (Human) antibody

Overview


	Antigen:	Cullin 2 (C-term.)
	Antibody Type:	Polyclonal
	Application:	Enzyme Immunoassay (EIA), Immunoprecipitation (IP), Western Blotting (WB)
	Reactivity:	Human
	Host:	Rabbit
	Quantity:	0.1 ml (85.0 mg/ml (by Refractometry))
	Price:	462,23 $ (Plus shipping costs and VAT, Invoice in EUR)
	Order number:	ABIN117948
	Availability:	Ships in 3 to 10 days

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Additional Information: Cullin 2 (C-term.) (Human) antibody

Antigen	Cullin 2 (C-term.)
Immunogen	Synthetic peptide corresponding to amino acids 733-745 of Human Cul2 (C-terminus) coupled to KLH.
Reactivity	Human
Antibody Type	Polyclonal
Format	Serum
Host	Rabbit

Application Details: Cullin 2 (C-term.) (Human) antibody

Application	Enzyme Immunoassay (EIA), Immunoprecipitation (IP), Western Blotting (WB)
Application Notes	This antibody reacts with human Cul2 by Western blot (1/500-1/1,000, An 86.9 kDa band corresponding to human Cul2 is detected. Most cell lines expressing Cul2 can be used as a positive control) and immunoprecipitation (The antibody immunoprecipitates in vitro translated product and protein from cell lysates (using HeLa or NIH-3T3)). ELISA (1/2,000-1/10,000). Other applications not tested. Optimal dilutions of this antibody are dependent on conditions and should be determined by the user. Purity and Specificity: This product is monospecific antiserum processed by delipidation and defibrination followed by sterile filtration. This product reacts with human Cullin 2. Cross reactivity with Cul2 from other sources has not been determined. Cullins assemble a potentially large number of ubiquitin ligases by binding to the RING protein ROC1 to catalyse polyubiquitination, as well as binding to various specificity factors to recruit substrates. Cullin 2 is an essential component of the SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complex, which mediates the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription. In the SCF complex, cul2 serves as a rigid scaffold that organizes the SKP1-F-box protein and RBX1 subunits. Cul2 may also contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. Cul2 is part of the SCF complex consisting of CUL1, RBX1, SKP1 and SKP2, where it interacts directly with SKP1, SKP2 and RBX1. Cul2 also interacts with RNF7 and is part of a complex with TIP120A/CAND1 and RBX1. The unneddylated form interacts with TIP120A/CAND1 and the interaction negatively regulates the association with SKP1 in the SCF complex.
Concentration	85.0 mg/ml (by Refractometry)
Buffer	This purified antibody is supplied as sterile filtered, liquid serum containing 0.01% (w/v) Sodium Azide as preservative.
Storage	Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. Conjugation pathways for ubiquitin and ubiquitin-like modifiers (UBLs). Most modifiers mature by proteolytic processing from inactive precursors (a, amino acid). Arrowheads point to the cleavage sites. Ubiquitin is expressed either as polyubiquitin or as a fusion with ribosomal proteins. Conjugation requires activating (E1) and conjugating (E2) enzymes that form thiolesters (S) with the modifiers. Modification of cullins by RUB involves SCF(SKP1/cullin-1/F-box protein) /CBC(cullin-2/elongin B/elonginC) -like E3 enzymes that are also involved in ubiquitination. In contrast to ubiquitin, the UBLs do not seem to form multi-UBL chains. UCRP(ISG15) resembles two ubiquitin moieties linked head-to-tail. Whether HUB1 functions as a modifier is currently unclear. APG12 and URM1 are distinct from the other modifiers because they are unrelated in sequence to ubiquitin. Data contributed by S.Jentsch, see references below. Protein Sequence: Human Cullin 2 (745 aa 86.9 kDa) 1 mslkprvvdf detwnklltt ikavvmleyv eratwndrfs diyalcvayp eplgerlyte 61 tkiflenhvr hlhkrvlese eqvlvmyhry weeyskgady mdclyrylnt qfikknklte 121 adlqygyggv dmneplmeig elaldmwrkl mveplqaili rmllreiknd rggedpnqkv 181 ihgvinsfvh veqykkkfpl kfyqeifesp fltetgeyyk qeasnllqes ncsqymekvl 241 grlkdeeirc rkylhpssyt kvihecqqrm vadhlqflha echniirqek kndmanmyvl 301 lravstglph miqelqnhih deglratsnl tqenmptlfv esvlevhgkf vqlintvlng 361 dqhfmsaldk altsvvnyre pksvckapel lakycdnllk ksakgmtene vedrltsfit 421 vfkyiddkdv fqkfyarmla krlihglsms mdseeamink lkqacgyeft sklhrmytdm 481 svsadlnnkf nnfiknqdtv idlgisfqiy vlqagawplt qapsstfaip qeleksvqmf 541 elfysqhfsg rkltwlhylc tgevkmnylg kpyvamvtty qmavllafnn setvsykelq 601 dstqmnekel tktikslldv kminhdseke didaessfsl nmnfsskrtk fkittsmqkd 661 tpqemeqtrs avdedrkmyl qaaivrimka rkvlrhnali qevisqsrar fnpsismikk 721 cievlidkqy iersqasade ysyva Specific References: Feng, H., Zhong, W., Punkosdy, G., Gu, S., Zhou, L., Seabolt,. E.K. and Kipreos, E.T. (1999) CUL-2 is required for the G1-to S-phase transition and mitotic chromosome condensation in Caenorhabditis elegans. Nat Cell Bio 1, 486-492. Kipreos,E.T., Lander,L.E., Wing,J.P., He,W.W. and Hedgecock,E.M. (1996) cul-1 is required for cell cycle exit in C. elegans and identifies a novel gene family. Cell 85 (6), 829-839. Michel,J.J. and Xiong,Y. (1998) Human CUL-1, but not other cullin family members, selectively interacts with SKP1 to form a complex with SKP2 and cyclin A. Cell Growth Differ. 9 (6), 435-449. Ohta,T., Michel,J.J., Schottelius,A.J. and Xiong,Y. (1999) ROC1, a homolog of APC11, represents a family of cullin partners with an associated ubiquitin ligase activity. Mol. Cell 3 (4), 535-541. Mathias, N., Johnson, S.L., Winey, M., Adams, A.E., Goetsch, L., Pringle, J.R., Byers, B., Goebl, M.G. (1996) Cdc53p acts in concert with Cdc4p and Cdc34p to control the G1-to-S-phase transition and identifies a conserved family of proteins. Mol Cell Biol 16:12, 6634-6643. Genereal References: Kamura,T., Sato,S., Iwai,K., Czyzyk-Krzeska,M., Conaway,R.C. and Conaway,J.W. (2000) Activation of HIF1alpha ubiquitination by a reconstituted von Hippel-Lindau (VHL) tumor suppressor complex. Proc. Natl. Acad. Sci. U.S.A. 97 (19), 10430-10435. Wada,H., Yeh,E.T. and Kamitani,T. (1999) Identification of NEDD8-conjugation site in human cullin-2. Biochem. Biophys. Res. Commun. 257 (1), 100-105. Pause,A., Lee,S., Worrell,R.A., Chen,D.Y., Burgess,W.H., Linehan,W.M. and Klausner,R.D. (1997) The von Hippel-Lindau tumor-suppressor gene product forms a stable complex with human CUL-2, a member of the Cdc53 family of proteins. Proc. Natl. Acad. Sci. U.S.A. 94 (6), 2156-2161. Jentsch S, Pyrowolakis G. (2000) Ubiquitin and its kin: how close are the family ties. Trends Cell Biol. 10(8):335-42.
Research Area	Apoptosis/Necrosis, Proteasome
Restrictions	Research only

Publications: Cullin 2 (C-term.) (Human) antibody

Publications

Feng, Zhong, Punkosdy et al.:
"CUL-2 is required for the G1-to-S-phase transition and mitotic chromosome condensation in Caenorhabditis elegans."
In: Nature cell biology , Vol. 1, Issue 8, pp. 486-92, 2000/01 (PubMed)

Weck, Pause, Pinzer:
"[Spinal meningioma as differential diagnosis of diabetic polyneuropathy]"
In: Deutsche medizinische Wochenschrift (1946) , Vol. 126, Issue 20, pp. 590-2, 2001/01 (PubMed)

Wada, Yeh, Kamitani:
"Identification of NEDD8-conjugation site in human cullin-2."
In: Biochemical and biophysical research communications , Vol. 257, Issue 1, pp. 100-5, 1999/01 (PubMed)

Images: Cullin 2 (C-term.) (Human) antibody

Conjugation pathways for ubiquitin and.,.ubiquitin-like modifiers (UBLs). Most modifiers mature by proteolytic processing from inactive precursors (a, amino acid). Arrowheads point to the cleavage sites. Ubiquitin is expressed either as polyubiquitin or as a fusion with ribosomal proteins. Conjugation requires activating (E1) and conjugating (E2) enzymes that form thiolesters (S) with the modifiers. Modification of cullins by RUB involves SCF(SKP1/cullin-1/F-box protein) /CBC(cullin-2/elongin B/elonginC) -like E3 enzymes that are also involved in ubiquitination. In contrast to ubiquitin, the UBLs do not seem to form multi-UBL chains. UCRP(ISG15) resembles two ubiquitin moieties linked head-to-tail. Whether HUB1 functions as a modifier is currently unclear. APG12 and URM1 are distinct from the other modifiers because they are unrelated in sequence to ubiquitin. Data contributed by S.Jentsch, see references below.

Image for Cullin 2 (C-term.) (Human) antibody (1)

External Information: Cullin 2 (C-term.) (Human) antibody

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