Cullin 3 (N-term.) (Human) antibody

Overview


	Antigen:	Cullin 3 (N-term.)
	Antibody Type:	Polyclonal
	Application:	Enzyme Immunoassay (EIA), Immunoprecipitation (IP), Western Blotting (WB)
	Reactivity:	Human, Mouse (Murine)
	Host:	Rabbit
	Quantity:	0.1 ml (85.0 mg/ml (by Refractometry))
	Price:	462,23 $ (Plus shipping costs and VAT, Invoice in EUR)
	Order number:	ABIN117949
	Availability:	Ships in 3 to 10 days

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Additional Information: Cullin 3 (N-term.) (Human) antibody

Antigen	Cullin 3 (N-term.)
Immunogen	Synthetic peptide corresponding to amino acids 1-12 of Human Cul3 (N-terminus) coupled to KLH.
Reactivity	Human, Mouse (Murine)
Antibody Type	Polyclonal
Format	Serum
Host	Rabbit

Application Details: Cullin 3 (N-term.) (Human) antibody

Application	Enzyme Immunoassay (EIA), Immunoprecipitation (IP), Western Blotting (WB)
Application Notes	This antibody reacts with human Cul3 by Western blot (1/500-1/1,000, An 88.9 kDa band corresponding to human Cul3 is detected, Most cell lines expressing Cul3 can be used as a positive control) and immunoprecipitation (The antibody immunoprecipitates in vitro translated product and protein from cell lysates (using HeLa or NIH-3T3)). ELISA (1/2,000-1/10,000). Other applications not tested. Optimal dilutions of this antibody are dependent on conditions and should be determined by the user. Purity and Specificity: This product is monospecific antiserum processed by delipidation and defibrination followed by sterile filtration. This product reacts with human Cullin 2. Cross reactivity with Cul2 from other sources has not been determined. Cullins assemble a potentially large number of ubiquitin ligases by binding to the RING protein ROC1 to catalyse polyubiquitination, as well as binding to various specificity factors to recruit substrates. Cullin 3 is an essential component of the SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complex, which mediates the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription. In the SCF complex, cul3 serves as a rigid scaffold that organizes the SKP1-F-box protein and RBX1 subunits. Cul3 may also contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. Unlike Cul1 and Cul2, Cul3 seems not to be a part of the SCF complex consisting of CUL1, RBX1, SKP1 and SKP2. Cul3 also interacts with RNF7 and is part of a complex with TIP120A/CAND1, Cyclin E and RBX1.
Concentration	85.0 mg/ml (by Refractometry)
Buffer	This purified antibody is supplied as sterile filtered, liquid serum containing 0.01% (w/v) Sodium Azide as preservative.
Storage	Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. Conjugation pathways for ubiquitin and ubiquitin-like modifiers (UBLs). Most modifiers mature by proteolytic processing from inactive precursors (a, amino acid). Arrowheads point to the cleavage sites. Ubiquitin is expressed either as polyubiquitin or as a fusion with ribosomal proteins. Conjugation requires activating (E1) and conjugating (E2) enzymes that form thiolesters (S) with the modifiers. Modification of cullins by RUB involves SCF(SKP1/cullin-1/F-box protein) /CBC(cullin-2/elongin B/elonginC) -like E3 enzymes that are also involved in ubiquitination. In contrast to ubiquitin, the UBLs do not seem to form multi-UBL chains. UCRP(ISG15) resembles two ubiquitin moieties linked head-to-tail. Whether HUB1 functions as a modifier is currently unclear. APG12 and URM1 are distinct from the other modifiers because they are unrelated in sequence to ubiquitin. Data contributed by S.Jentsch, see references below. Protein Sequence: Human Cullin 3 (768 aa 88.9 kDa) 1 msnlskgtgs rkdtkmrira fpmtmdekyv nsiwdllkna iqeiqrknns glsfeelyrn 61 aytmvlhkhg eklytglrev vtehlinkvr edvlnslnnn flqtlnqawn dhqtamvmir 121 dilmymdrvy vqqnnvenvy nlgliifrdq vvrygcirdh lrqtlldmia rerkgevvdr 181 gairnacqml milglegrsv yeedfeapfl emsaeffqme sqkflaensa svyikkvear 241 ineeiervmh cldksteepi vkvverelis khmktiveme nsglvhmlkn gktedlgcmy 301 klfsrvpngl ktmcecmssy lreqgkalvs eegegknpvd yiqglldlks rfdrfllesf 361 nndrlfkqti agdfeyflnl nsrspeylsl fiddklkkgv kglteqevet ildkamvlfr 421 fmqekdvfer yykqhlarrl ltnksvsdds eknmisklkt ecgcqftskl egmfrdmsis 481 nttmdefrqh lqatgvslgg vdltvrvltt gywptqsatp kcnippaprh afeifrrfyl 541 akhsgrqltl qhhmgsadln atfygpvkke dgsevgvgga qvtgsntrkh ilqvstfqmt 601 ilmlfnnrek ytfeeiqqet diperelvra lqslacgkpt qrvltkepks keienghift 661 vndqftsklh rvkiqtvaak qgesdperke trqkvdddrk heieaaivri mksrkkmqhn 721 vlvaevtqql karflpspvv ikkrieglie reylartped rkvytyva
Research Area	Apoptosis/Necrosis, Proteasome
Restrictions	Research only

Publications: Cullin 3 (N-term.) (Human) antibody

Publications

Furukawa, He, Borchers et al.:
"Targeting of protein ubiquitination by BTB-Cullin 3-Roc1 ubiquitin ligases."
In: Nature cell biology , Vol. 5, Issue 11, pp. 1001-7, 2003/01 (PubMed)

Chuang, Huang, Hsieh et al.:
"Sporadic paediatric and adult Burkitt lymphomas share similar phenotypic and genotypic features."
In: Histopathology , Vol. 52, Issue 4, pp. 427-35, 2008/01 (PubMed)

Images: Cullin 3 (N-term.) (Human) antibody

Conjugation pathways for ubiquitin and.,.ubiquitin-like modifiers (UBLs). Most modifiers mature by proteolytic processing from inactive precursors (a, amino acid). Arrowheads point to the cleavage sites. Ubiquitin is expressed either as polyubiquitin or as a fusion with ribosomal proteins. Conjugation requires activating (E1) and conjugating (E2) enzymes that form thiolesters (S) with the modifiers. Modification of cullins by RUB involves SCF(SKP1/cullin-1/F-box protein) /CBC(cullin-2/elongin B/elonginC) -like E3 enzymes that are also involved in ubiquitination. In contrast to ubiquitin, the UBLs do not seem to form multi-UBL chains. UCRP(ISG15) resembles two ubiquitin moieties linked head-to-tail. Whether HUB1 functions as a modifier is currently unclear. APG12 and URM1 are distinct from the other modifiers because they are unrelated in sequence to ubiquitin. Data contributed by S.Jentsch, see references below.

Image for Cullin 3 (N-term.) (Human) antibody (1)

External Information: Cullin 3 (N-term.) (Human) antibody

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