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Product details for Cyclin A (Human) antibody
Cyclin A (Human) antibody
Overview
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| Antigen: | Cyclin A |
| Antibody Type: | Monoclonal |
| Application: | Western Blotting (WB) |
| Reactivity: | Human, Mouse (Murine), Rat |
| Host: | Mouse |
| Quantity: | |
| Price: | 259,66 $ (Plus shipping costs , $13.59 dry ice fee and VAT, Invoice in EUR) |
| Order number: | ABIN131744 |
| Availability: | 7 to 10 days until shipment |
Additional Information: Cyclin A (Human) antibody
| Antigen | Cyclin A |
| Immunogen | This antibody was purified from hybridoma (clone E23.1) supernatant using protein A agarose. This hybridoma was established by fusion of mouse myeloma cell Sp2 with Balb/c mouse splenocyte immunized with bovine recombinant Cyclin A. |
| Reactivity | Human, Mouse (Murine), Rat |
| Antibody Type | Monoclonal |
| Isotype | IgG2a »Matching secondary antibodies |
| Description | Cyclin A is a 60 kDa cyclin that binds both cdk2 and p34cdc2 (cdk1), giving two distinct cyclin A kinase activities, one appearing in G1/S phase, the other in G2/M. The level of cyclin A protein peaks in S-phase, and the activity of the cdk2-cyclin A complexes is essential for DNA replication and progression through S phase. Cyclin A is thus a marker for actively proliferating cells and for cells in S-phase. In normal cells, cdk2-cyclin A complexes associate with PCNA and p21Cip1/WAF1. In addition, cyclin A has been implicated in cell transformation and is found in complexes with E1A, transcription factors DRT F1 and E2F, and retinoblastoma protein, p110. |
| Clone | E23-1 |
| Host | Mouse |
| Specificity | This antibody reacts with Cyclin A (55 kDa) on Western blotting. |
Application Details: Cyclin A (Human) antibody
| Application | Western Blotting (WB) |
| Application Notes | SDS-PAGE & Western Blotting 1) Wash the cells 3 times with PBS and suspend with 10 volumes of cold Lysis buffer (50 mM Tris-HCl, pH 7.2, 250 mM NaCl, 0.1% NP-40, 2 mM EDTA, 10% glycerol) containing appropriate protease inhibitors. Incubate it at Western blot analysis of Cyclin A expression in Jurkat cells (1), Raji cells (2), HeLa cells (3), WR19L cells (4) and PC12 cells (5) using Western blotting: 1 µg / mL for chemiluminescence detection system. Immunoprecipitation: Not recommended. Immunohistochemistry: Not tested. Immunocytochemistry: Not recommended. Flow Cytometry: Not tested. |
| Concentration | 1 mg/mL |
| Buffer | 100 µg IgG in 100 µL volume of PBS containing 50% glycerol, pH 7.2. Contains no preservatives. |
| Research Area | Cell Cycle |
| Restrictions | For Research Use Only |
Publications: Cyclin A (Human) antibody
| Publications |
Geley, Kramer, Gieffers et al.: "Anaphase-promoting complex/cyclosome-dependent proteolysis of human cyclin A starts at the beginning of mitosis and is not subject to the spindle assembly checkpoint." In: The Journal of cell biology , Vol. 153, Issue 1, pp. 137-48, 2001/01 (PubMed) |
Images: Cyclin A (Human) antibody
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Western blot analysis of Cyclin A expression in Jurkat cells (1), Raji cells (2), HeLa cells (3), WR19L cells (4) and PC12 cells (5) using ABIN131744 |
External Information: Cyclin A (Human) antibody
| Google Scholar | Find more references for clone E23-1 on Google Scholar™ |
| EMBL Harvester | Search human, mouse or rat genome for antigen Cyclin A (Bioinformatic Harvester (EMBL)) |
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