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Product details for Cyclin B1 (Human) antibody
Cyclin B1 (Human) antibody
Overview
| Antigen: | Cyclin B1 |
| Antibody Type: | Monoclonal |
| Application: | Western Blotting (WB) |
| Reactivity: | Human |
| Host: | Mouse |
| Quantity: | |
| Price: | 342,59 $ (Plus shipping costs and VAT, Invoice in EUR) |
| Order number: | ABIN145082 |
| Availability: | 7 to 10 days until shipment |
Additional Information: Cyclin B1 (Human) antibody
| Antigen | Cyclin B1 |
| Immunogen | Recombinant protein corresponding to human Cyclin B1. |
| Reactivity | Human |
| Antibody Type | Monoclonal |
| Format | Liquid |
| Isotype | IgG1 »Matching secondary antibodies |
| Description | In mammals, cyclin B associates with inactive p34 cdc2 which facilitates phosphorylation of p34 cdc2 at aa 14 Thr and 15 Tyr. This maintains the inactive state until the end of G2 phase. The inactive cyclin B p34 cdc2 complex continues to accumulate in the cytoplasm until the completion of DNA synthesis, when Cdc25, a specific protein phosphatase, dephosphorylates aa 14Thr and 15Tyr of p34 cdc2 rendering the complex active at the G2 / M boundary. This mitotic kinase complex remains active until the metaphase / anaphase transition when cyclin B is degraded. This degradation process is ubiquitin dependent and is necessary for the cell to exit mitosis. Therefore, cyclin B p34 cdc2 plays a critical role in G2 to M transition. |
| Clone | GNS1 |
| Host | Mouse |
Application Details: Cyclin B1 (Human) antibody
| Application | Western Blotting (WB) |
| Application Notes | WB: Use at a dilution of 1/500-1/1000. Predicted molecular weight: 58 kDa. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user. |
| Purity | Tissue culture supernatant |
| Buffer | Preservative: 0.1% Sodium Azide, 0.2% Gelatin, 0.01% ThimerosalConstituents: PBS, Tissue Culture Supernatant |
| Storage | Store at 4 C. Aliquot and store at -20 C long-term. |
| Research Area | Cell Cycle |
| Restrictions | For research use only |
External Information: Cyclin B1 (Human) antibody
| Google Scholar | Find more references for clone GNS1 on Google Scholar™ |
| EMBL Harvester | Search human, mouse or rat genome for antigen Cyclin B1 (Bioinformatic Harvester (EMBL)) |
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