Cyclin B1 (Human) antibody

Overview


	Antigen:	Cyclin B1
	Antibody Type:	Polyclonal
	Application:	Immunohistochemistry (IHC), ELISA, Western Blotting (WB), Immunoprecipitation (IP)
	Reactivity:	Bovine, Human, Mouse (Murine), Rat, Sheep (Ovine)
	Host:	Rabbit
	Quantity:	100ul
	Price:	338,33 $ (Plus shipping costs and VAT, Invoice in EUR)
	Order number:	ABIN97948
	Availability:	7 days until shipment

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Additional Information: Cyclin B1 (Human) antibody

Antigen	Cyclin B1
Immunogen	Full length GST fusion protein corresponding to the human gene..
Reactivity	Bovine, Human, Mouse (Murine), Rat, Sheep (Ovine)
Antibody Type	Polyclonal
Format	Liquid (sterile filtered)
Host	Rabbit

Application Details: Cyclin B1 (Human) antibody

Application	Immunohistochemistry (IHC), ELISA, Western Blotting (WB), Immunoprecipitation (IP)
Application Notes	Dilution: This product was assayed by immunoblot and found to be reactive against Cyclin B1 at a dilution of 1/500 to 1/1000 followed by reaction with Peroxidase conjugated Affinity Purified antiRabbit IgG [HampL] [Goat] code 6111302. AntiCyclin B1 is suitable for the detection by immunoblot of human rat and mouse Cyclin B1. Suggested dilutions for ELISA are 1/500 to 1/2000. Optimal titers for other applications should be determined by the researcher. Notice: suitable for ELISA immunoprecipitation immunoblotting and other immunological methods requiring high titer and specificity.
Purity	NoneStabilizer: None Preservative: 0.01% (w/v) Sodium Azide Figure 1. Western blot analysis is shown using Anti-Cyclin B1 antibody to detect Human Cyclin B1 present in asynchronous HN30 cell lysates. HN30 cells, are from head and neck cancer cells that over express cyclin B1 and D1. Comparison to a molecular weight marker indicates a band of ~62 kDa corresponding to the expected molecular weight for the protein (arrowhead). The blot was incubated with a 1dilution of the antibody at room temperature. Detection occurred using a 1:10,000 of HRP conjugated Goat-a-Rabbit IgG ABIN102010 and chemiluminescence reagent with a 1-min exposure time. Other detection systems will yield similar results. Personal communication Luca Cote. :500
Purification	This product was prepared from monospecific antiserum by delipidation and defibrination. Antiserum will specifically react with a 62 kDa Cyclin B1 protein from human, rat and mouse tissue. No reaction was observed against other related cyclins. Cross reactivity with Cyclin B1 from other species may also occur.
Storage	Store vial at -20° C or below prior to opening. Dilute only prior to immediate use. Aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is six (6) months from date of opening product.
Research Area	Cell Cycle
Restrictions	For Research Use only

Images: Cyclin B1 (Human) antibody

Western blot analysis is shown using antibodies-online's Anti-Cyclin B1 antibody to detect Human Cyclin B1 present in asynchronous HN30 cell lysates. HN30 cells, are from head and neck cancer cells that over express cyclin B1 and D1. Comparison to a molecular weight marker indicates a band of ~62 kDa corresponding to the expected molecular weight for the protein (arrowhead). The blot was incubated with a 1dilution of the antibody at room temperature. Detection occurred using a 1:10,000 of HRP conjugated Goat-a-Rabbit IgG ABIN102010 and chemiluminescence reagent with a 1-min exposure time. Other detection systems will yield similar results. Personal communication Luca Cote.

Image for Cyclin B1 (Human) antibody (1)

External Information: Cyclin B1 (Human) antibody

Google Scholar	Find more references for antigen Cyclin B1 on Google Scholar™
EMBL Harvester	Search human, mouse or rat genome for antigen Cyclin B1 (Bioinformatic Harvester (EMBL))