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Product details for Cyclin D1 (Human) antibody
Cyclin D1 (Human) antibody
Overview
| Antigen: | Cyclin D1 |
| Antibody Type: | Monoclonal |
| Application: | Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunoprecipitation (IP), Western Blotting (WB) |
| Reactivity: | Dog (Canine), Guinea Pig, Human, Monkey, Mouse (Murine), Rabbit, Rat |
| Host: | Mouse |
| Quantity: | |
| Price: | 312,68 $ (Plus shipping costs and VAT, Invoice in EUR) |
| Order number: | ABIN111000 |
| Availability: | 3 to 10 days until shipment |
Additional Information: Cyclin D1 (Human) antibody
| Antigen | Cyclin D1 |
| Immunogen | Human recombinant full-length cyclin D1 protein |
| Reactivity | Dog (Canine), Guinea Pig, Human, Monkey, Mouse (Murine), Rabbit, Rat |
| Antibody Type | Monoclonal |
| Format | Purified |
| Isotype | IgG2a »Matching secondary antibodies |
| Clone | DCS-6 |
| Host | Mouse |
| Specificity | DCS-6 reacts specifically with cyclin D1 protein present predominantly in G1 phase of cell cycle. Does not cross-react with D2 or D3 cyclins (the closest relatives within the cyclin family). DCS-6 is especially useful in tumor diagnosis: it can be used as fast method to identify cases with 11q13 amplifications. Cyclin D1 gene is identical to the BCL-1 and the PRAD-1 oncogene and is part of the chromosome 11q13 amplicon which is amplified in several common tumor types like breast, lung, esophagus, urinary bladder and head and neck carcinoma. Polypeptide reacting: Mr 36 000 polypeptide (cyclin D1 polypeptide of human cells). Reacts with MCF-7, MDA-MB-134 (breast cancer cell lines), UMSC22 (squamous cell carcinoma cell line), U-2-OS (osteosarcoma cell line) RT 112, A 431, HT 29 (for additional cell lines and their reaction patterns see ref. 1). Species reactivity: Human, monkey, rat, mouse, dog, and rodents. |
Application Details: Cyclin D1 (Human) antibody
| Application | Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunoprecipitation (IP), Western Blotting (WB) |
| Application Notes | Immunostaining of frozen and paraffin-embedded tissue and culture cells (1/10, Paraffin without microwave treatment only after fixation in methacarn, ethanol or phenol formalin, weak reaction using routine formol-fixed paraffin sections, Paraffin with microwave treatment for routine formalin-fixation). Immunoblotting (Western Blot). Immunoprecipitation. Microinjection/in vitro neutralization of cyclin D1 function. Flow cytometry. Other applications not tested. Optimal dilutions of this antibody are dependent on conditions and should be determined by the user. |
| Buffer | This antibody is supplied as Protein A purified IgG fraction lyophilised from PBS pH 7.4 with 0.5% BSA and 0.09% sodium azide as preservative. Reconstitute with 1.0 ml dist. water. |
| Storage | Store the antibody at 4-8°C for one month or at –20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch. |
| Research Area | Cell Cycle |
| Restrictions | For Research Use only |
External Information: Cyclin D1 (Human) antibody
| Google Scholar | Find more references for clone DCS-6 on Google Scholar™ |
| EMBL Harvester | Search human, mouse or rat genome for antigen Cyclin D1 (Bioinformatic Harvester (EMBL)) |
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