BASKET
HELP
Product details for Cyclin D1 (Human) antibody
Cyclin D1 (Human) antibody
Overview
|
|
| Antigen: | Cyclin D1 |
| Antibody Type: | Monoclonal |
| Application: | Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC) |
| Reactivity: | Human, Mouse (Murine), Rat |
| Host: | Mouse |
| Quantity: | |
| Price: | 69,33 $ (Plus shipping costs , $13.59 dry ice fee and VAT, Invoice in EUR) |
| Order number: | ABIN131614 |
| Availability: | 7 to 10 days until shipment |
Additional Information: Cyclin D1 (Human) antibody
| Antigen | Cyclin D1 |
| Immunogen | This antibody was purified from hybridoma (clone DCS-6) supernatant using protein-A Sepharose. This hybridoma was established by fusion of mouse myeloma cell NS-2 with Balb/c mouse splenocyte immunized with gel-purified full-length recombinant human cyclin D1 protein. |
| Reactivity | Human, Mouse (Murine), Rat |
| Antibody Type | Monoclonal |
| Isotype | IgG2ak »Matching secondary antibodies |
| Description | Cyclin D1, also known as Bcl-1, CCND1, or PRAD-1, is a 36 kDa nuclear protein that plays a key regulatory role during the G1 phase of the cell cycle by regulating the activity of Cdk proteins. Cyclin D1 forms a complex with Cdk4, then binds to and phosphorylates Rb protein, triggering cells to progress from G0/G1 to S and thus driving cellular proliferation. Cyclin D1 protein overexpression is found in the majority of human breast cancers and is usually linked with poor prognosis. Overexpression of cyclin D1 is a well-established criterion for the diagnosis of Mantle Cell Lymphoma, and also has been used to distinguish malignant breast carcinomas from premalignant breast lesions. |
| Clone | DCS-6 |
| Host | Mouse |
| Specificity | This antibody reacts with human, mouse and rat cyclin D1. |
Application Details: Cyclin D1 (Human) antibody
| Application | Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC) |
| Application Notes | Western blotting: 1-5 µg/mL. Immunoprecipitation: 3 µg / 200-300 µL of cell extract. Immunohistochemistry: 1-5 µg/mL Heat treatment is necessary for paraffin embedded sections. Microwave oven: 2 times for 10 minutes each in citrate buffer (pH 6.5). 1234 94 67 43 30 20 kDa. Western blot analysis of cyclin D1 expression in ZR75-1 cells (1), C2C12 cells (2), NIH/3T3 cells (3) and Rat-1 cells (4) using K0062-3. Immunoprecipitation of cyclin D1 from ZR75-1 cells with normal mouse IgG (1) or K0062-3 (2). After immunoprecipitated with the antibody, immunocomplex was resolved on SDS-PAGE and immunoblotted with K0062-3. 12 94 67 43 30 20 kDa Cyclin D1 IgG heavy chain Immunohistochemical detection of cyclin D1 on ZR75-1 paraffin embedded section with K0062-3. |
| Concentration | 1 mg/mL |
| Buffer | 100 µg IgG in 100 µL volume of PBS containing 50% glycerol, pH 7.2. No preservative is contained. |
| Research Area | Cell Cycle |
| Restrictions | For Research Use Only |
Publications: Cyclin D1 (Human) antibody
| Publications |
Bartkova, Lukas, Strauss et al.: "Cell cycle-related variation and tissue-restricted expression of human cyclin D1 protein." In: The Journal of pathology , Vol. 172, Issue 3, pp. 237-45, 1994/01 (PubMed) |
Images: Cyclin D1 (Human) antibody
|
Immunohistochemical detection of cyclin D1 on ZR75-1 paraffin embedded section with ABIN131613
Western blot analysis of cyclin D1 expression in ZR75-1 cells (1), C2C12 cells (2), NIH/3T3 cells (3) and Rat-1 cells (4) using ABIN131613
Immunoprecipitation of cyclin D1 from ZR75-1 cells with normal mouse IgG (1) or ABIN131613(2). After immunoprecipitated with the antibody, immunocomplex was resolved on SDS-PAGE and immunoblotted with ABIN131613 |
External Information: Cyclin D1 (Human) antibody
| Google Scholar | Find more references for clone DCS-6 on Google Scholar™ |
| EMBL Harvester | Search human, mouse or rat genome for antigen Cyclin D1 (Bioinformatic Harvester (EMBL)) |
browse antibodies by
Antigens
Secondary Antibodies
Research areas
Clones
Hosts
Applications
Reactivity
Supplier
Antigens
Secondary Antibodies
Research areas
Clones
Hosts
Applications
Reactivity
Supplier
