Western blot analysis is shown using antibodies-online's Anti-Cyclin D1 antibody to detect Human Cyclin D1 present in asynchronous HN30 cell lysates. HN30 cells, are from head and neck cancer cells that over express cyclin B1 and D1. Comparison to a molecular weight marker indicates a band of ~34 kDa corresponding to the expected molecular weight for the protein (arrowhead). The blot was incubated with a 1:500 dilution of the antibody at room temperature. Detection occurred using a 1:10,000 of HRP conjugated Goat-a-Rabbit IgG ABIN102010 and chemiluminescence reagent with a 1-min exposure time. Other detection systems will yield similar results. Personal communication Luca Cote.

A model summarizes the interactions between cyclins and the cyclin dependent kinases in multicellular animal cells. The R point is the restriction point. The diagram shows the stages of the cell cycle and the binding of the specified cyclins with the corresponding CDKs at each stage. cdc2 is kinase, p107 and E2F are proteins involved in transcription.