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Product details for Cyclin D1 (Human) antibody
Cyclin D1 (Human) antibody
Overview
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| Antigen: | Cyclin D1 |
| Antibody Type: | Monoclonal |
| Application: | Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) |
| Reactivity: | Human |
| Host: | Mouse |
| Quantity: | |
| Price: | 591,43 $ (Plus shipping costs and VAT, Invoice in EUR) |
| Order number: | ABIN97959 |
| Availability: | 7 days until shipment |
Additional Information: Cyclin D1 (Human) antibody
| Antigen | Cyclin D1 |
| Immunogen | BALB/C mice were immunized with human recombinant cyclin D1 protein. |
| Reactivity | Human |
| Antibody Type | Monoclonal |
| Format | Supernatant |
| Isotype | IgG2a Kappa »Matching secondary antibodies |
| Clone | DCS-6 |
| Host | Mouse |
| Specificity | This antibody is specific to human cyclin D1. Cyclin D1 gene is located on 11q13 and has been shown to be amplified in a variety of tumors. Maximum expression of cyclin D1 occurs at a critical point in mid to late G1 phase of the cell cycle. This antibody cross reacts with monkey, rat and mouse. |
Application Details: Cyclin D1 (Human) antibody
| Application | Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) |
| Application Notes | Titer/Working Dilution: This antibody may be diluted to a titer of 1:25-1:50 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Breast carcinoma. Cellular localization: Nuclear Other Applications: Immunoprecipitation (1:50), Western Blotting (1:50 to 1:100) |
| Buffer | This antibody is supplied as purified immunoglobulin fraction containing sodium azide as preservative. |
| Storage | Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label. |
| Research Area | Cell Cycle |
| Restrictions | For Research Use only |
Images: Cyclin D1 (Human) antibody
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Formalin fixed paraffin embedded human breast carcinoma stained with Cyclin D1 antibody |
External Information: Cyclin D1 (Human) antibody
| Google Scholar | Find more references for clone DCS-6 on Google Scholar™ |
| EMBL Harvester | Search human, mouse or rat genome for antigen Cyclin D1 (Bioinformatic Harvester (EMBL)) |
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