Cyclin D2 (Human) antibody

Overview


	Antigen:	Cyclin D2
	Antibody Type:	Monoclonal
	Application:	Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC)
	Reactivity:	Human, Mouse (Murine)
	Host:	Mouse, Rat
	Quantity:	100 µg (1 mg/mL) (Product variants)
	Price:	273,26 $ (Plus shipping costs , $13.59 dry ice fee and VAT, Invoice in EUR)
	Order number:	ABIN131617
	Availability:	7 to 10 days until shipment

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Additional Information: Cyclin D2 (Human) antibody

Antigen	Cyclin D2
Immunogen	This antibody was purified from hybridoma (clone DCS-5) supernatant using protein-A Sepharose. This hybridoma was established by fusion of mouse myeloma cell NS-2 with Balb/c mouse splenocyte immunized with the full-length recombinant human cyclin D2 protein.
Reactivity	Human, Mouse (Murine)
Antibody Type	Monoclonal
Isotype	IgG2b »Matching secondary antibodies
Description	Cyclin D2 (34 kDa) is one of three D-type cyclins that are synthesized in G 1 phase and induced in response to agents that promote re-entry into the cell cycle. D-type cyclins assemble with cyclin-dependent kinases Cdk4 and Cdk6 to form complexes that phosphorylate key substrates, including the retinoblastoma protein Rb, involved in proliferation. Generally, D cyclins appear late in the G 1 phase of the cell cycle, however, cyclin D2 accumulates in Go and at the G 1 /S interface. A role for cyclin D2 has been suggested in differentiation and oncogenic transformation. Cyclin D2 is expressed in macrophages in response to CSF-1 stimulation, and overexpression of cyclin D2 is associated with increased in vivo invasiveness of human squamous carcinoma cells. For research use only. Not for clinical diagnosis. 94 67 43 30 20 kDa Western blot analysis of cyclin D2 expression in NIH/3T3 cells using K0064-3. 14
Clone	DCS-5
Host	Mouse, Rat
Specificity	This antibody reacts with human and mouse Cyclin D2.

Application Details: Cyclin D2 (Human) antibody

Application	Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC)
Application Notes	Western blotting: 1-5 µg/mL. Immunoprecipitation: 2 µg / 200-300 µL of cell extract Immunohistochemistry: Not recommended.
Concentration	1 mg/mL
Buffer	100 µg IgG in 100 µL volume of PBS containing 50% glycerol, pH 7.2. No preservative is contained. 94 67 43 30 20 kDa Immunoprecipitation of cyclin D2 from NIH/3T3 cells with normal mouse IgG (1) or K0064-3 (2). After immunoprecipitated with the antibody,
Research Area	Cell Cycle
Restrictions	For Research Use Only

Publications: Cyclin D2 (Human) antibody

Publications

Lukas, Bartkova, Welcker et al.:
"Cyclin D2 is a moderately oscillating nucleoprotein required for G1 phase progression in specific cell types."
In: Oncogene , Vol. 10, Issue 11, pp. 2125-34, 1995/01 (PubMed)

Boonen, van Oirschot, van Diepen et al.:
"Cyclin D3 regulates proliferation and apoptosis of leukemic T cell lines."
In: The Journal of biological chemistry , Vol. 274, Issue 49, pp. 34676-82, 2000/01 (PubMed)

Images: Cyclin D2 (Human) antibody

Western blot analysis of cyclin D2 expression in NIH/3T3 cells using ABIN131617.

Image for Cyclin D2 (Human) antibody (1)

Immunoprecipitation of cyclin D2 from NIH/3T3 cells with normal mouse IgG(1) or ABIN131617 (2). After immunoprecipitated with the antibody, immunocomplexwas resolved on SDS-PAGE and immunoblottedwith ABIN131617.

Image for Cyclin D2 (Human) antibody (2)

Product variants

Cyclin D2 (Human) antibody (Quantity: 10 µg/50 µl)

External Information: Cyclin D2 (Human) antibody

Google Scholar	Find more references for clone DCS-5 on Google Scholar™
EMBL Harvester	Search human, mouse or rat genome for antigen Cyclin D2 (Bioinformatic Harvester (EMBL))