SHOW TUTORIAL VIDEO Show Tutorial Video
Product details for  Cyclin D3 (Human) antibody
Print Version Print Version       Download Datasheet Download Datasheet       Back to search results Back to search results       Add to basket Add to basket

Cyclin D3 (Human) antibody

Overview

Antigen: Cyclin D3
Antibody Type: Monoclonal
Application: Western Blotting (WB), Immunohistochemistry (IHC)
Reactivity: Human, Mouse (Murine), Rat
Host: Mouse, Rat
Quantity: 200 µl (Product variants)
Price: 259,66 $   (Plus shipping costs , $13.59 dry ice fee and VAT, Invoice in EUR)
Order number: ABIN131547
Availability: 7 to 10 days until shipment
Hints on ordering Hints on ordering
Add to basket

on this page

Additional Information
Application Details
Publications
Related Products
External Information

Customer Service

Technical Inquiry
Send product details to colleague
Download Datasheet

Additional Information:  Cyclin D3 (Human) antibody

back to top ^


Antigen Cyclin D3
Immunogen This antibody was concentrated from hybridoma (clone DCS-22) supernatant. This hybridoma was established by fusion of mouse myeloma cell NS-2 with BALB/c mouse splenocyte immunized with the recombinant fusion protein corresponding to the full length human cyclin D3 molecule.
Reactivity Human, Mouse (Murine), Rat
Antibody Type Monoclonal
Format Liquid
Isotype IgG1k »Matching secondary antibodies
Clone DCS-22
Host Mouse, Rat
Specificity This antibody reacts with human cyclin D3 antigen. No crossreaction with recombinant human cyclin D1 or cyclin D2 proteins.

Application Details:  Cyclin D3 (Human) antibody

back to top ^


Application Western Blotting (WB), Immunohistochemistry (IHC)
Application Notes Immunohistochemical staining for paraffin-embedded sections: SAB method 1) Deparaffinize the sections with Xylene 3 times for 3-5 minutes each. 2) Wash the slides with Ethanol 3 times for 3-5 minutes each. 3) Wash the slides with PBS 3 times for 3-5 minutes each. 4) Heat treatment Heat treatment by Autoclave:Place the slides put on staining basket in 500 mL beaker with 500 mL citrate buffer (pH 6.5). Cover the beaker with plastic wrap, then process the slides with the autoclave for 10 minutes at 110°C. Let the slides cool down in the beaker at room temperature for about 40 minutes. Heat treatment by microwave oven:Place the slides put on staining basket in 500 mL beaker with 500 mL citrate buffer (pH 6.5). Cover the beaker with plastic wrap, then process the slides 2 times for 10 minutes each at 500 W with micro wave oven. Let the slides cool down in the beaker at room temperature for about 40 minutes. 5) Remove the slides from the citrate buffer and cover each section with 3% H 2 O 2 for 10 minutes at room temperature to block endogenous peroxidase activity. Wash 3 times in PBS for 5 minutes each. 6) Remove the slides from PBS, wipe gently around each section and cover tissues with Protein Blocking Agent (Ultratech HRP Kit, IMMUNOTECH Cat# IM-2391) for 5 minutes to block non-specific antibody staining. Do not wash. 7) Tip off the blocking buffer, wipe gently around each section and cover tissues with the cyclin D3 monoclonal antibody diluted with PBS containing 1% BSA (1:20 - 1:40). 8) Incubate the sections for 1 hour at room temperature. 9) Wash the slides 3 times in PBS for 5 minutes each. 10) Wipe gently around each section and cover tissues with Polyvalent Biotinylated antibody (Ultratech HRP Kit). Incubate for 10 minutes at room temperature. Wash as in step 9). Immunohistochemistry: 1:20 - 1:40 Heat treatment is necessary for paraffin embedded sections. Autoclave: 10 minutes in citrate buffer (pH 6.5) at 110°C Microwave oven: 2 times for 10 minutes each in citrate buffer (pH 6.5) More strong staining intensity may be shown by autoclave than microwave oven.. Western blotting: 1:100 - 1:200 Research Applications.
Buffer 200 µ L aliquot of concentrate antibody from the supernatant with preservative (0.1% sodium azide) *Azide may react with copper or lead in plumbing system to form explosive metal azides. Therefore, always flush plenty of water when disposing materials con
Research Area Cell Cycle
Restrictions For Research Use Only

Publications:  Cyclin D3 (Human) antibody

back to top ^


Publications Bartkova, Zemanova, Bartek:
"Abundance and subcellular localisation of cyclin D3 in human tumours."
In: International journal of cancer. Journal international du cancer , Vol. 65, Issue 3, pp. 323-7, 1996/01 (PubMed)

Depoortere, Van Keymeulen, Lukas et al.:
"A requirement for cyclin D3-cyclin-dependent kinase (cdk)-4 assembly in the cyclic adenosine monophosphate-dependent proliferation of thyrocytes."
In: The Journal of cell biology , Vol. 140, Issue 6, pp. 1427-39, 1998/01 (PubMed)

Depoortere, Pirson, Bartek et al.:
"Transforming growth factor beta(1) selectively inhibits the cyclic AMP-dependent proliferation of primary thyroid epithelial cells by preventing the association of cyclin D3-cdk4 with nuclear p27(kip1)."
In: Molecular biology of the cell , Vol. 11, Issue 3, pp. 1061-76, 2000/01 (PubMed)

External Information:  Cyclin D3 (Human) antibody

back to top ^


Google Scholar Find more references for clone DCS-22 on Google Scholar™
EMBL Harvester Search human, mouse or rat genome for antigen Cyclin D3 (Bioinformatic Harvester (EMBL))