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Cyclin D3 (Human) antibody

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Image for Cyclin D3 (Human) antibody
Antigen: Cyclin D3
Antibody Type: Monoclonal
Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)
Reactivity: Human, Mouse (Murine), Rat
Host: Mouse
Quantity: 100 µg (1 mg/mL)
Price: 239,27 $   (Plus shipping costs , $13.59 dry ice fee and VAT, Invoice in EUR)
Order number: ABIN131548
Availability: 7 to 10 days until shipment
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Additional Information:  Cyclin D3 (Human) antibody

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Antigen Cyclin D3
Immunogen This antibody was concentrated from hybridoma (clone DCS-22) supernatant. This hybridoma was established by fusion of mouse myeloma cell NS-2 with Balb/c mouse splenocyte immunized with the recombinant fusion protein corresponding to the full length human cyclin D3 molecule.
Reactivity Human, Mouse (Murine), Rat
Antibody Type Monoclonal
Format Liquid
Isotype IgG1., IgG1k »Matching secondary antibodies
Description Cyclin D3 is one of three D-type cyclins that are synthesized in G 1 phase and induced in response to agents that promote re-entry into the cell cycle. D-type cyclins assemble with cyclin-dependent kinases CDK4 and CDK6 to form complexes that phosphorylate key substrates, including Rb, involved in proliferation. Expression of cyclin D3 occurs late in the G1 phase of the cell cycle. Unlike cyclins D2 and D1, cyclin D3 is not induced by CSF-1 stimulation in macrophages. Cyclin D3 has been shown to associate with PCNA (Proliferating Cell Nuclear Antigen) and p21, suggesting a possible role for cyclin D3 in DNA replication and repair.
Clone DCS-22
Host Mouse
Specificity This antibody reacts with human cyclin D3 antigen. No crossreaction with recombinant human cyclin D1 or cyclin D2 proteins.

Application Details:  Cyclin D3 (Human) antibody

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Application Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)
Application Notes Western blotting: 1 µg/mL. Immunoprecipitation: 2 µg of /200-300µL of cell extract. Immunohistochemistry: 5 µg/mL Heat treatment is necessary for paraffin embedded sections. Microwave oven: 2 times for 10 minutes each in citrate buffer (pH 6.5). 94 67 43 30 20 14 kDa 94 67 43 30 20 kDa. Western blot analysis of Cyclin D3 expression in MCF7 cells (A), Rat-1 cells (B-1) and NIH/3T3 cells (B-2) using K0013-3. A B 12 Immunohistochemical detection of Cyclin D3 on paraffin embedded section of human tonsil with K0013-3. Clear nuclear staining was shown in germinal center cells of human tonsil. 94 67 43 30 kDa 12. Immunoprecipitation of Cyclin D3 from MCF7 cells with normal mouse IgG (1) or K0013-3 (2). After immunoprecipitated with the antibody, immunocomplex was resolved on SDS-PAGE and immunoblotted with K0013-3. IgG heavy chain Cyclin D3
Concentration 1 mg/mL
Buffer 100 µg IgG in 100 µL volume of PBS containing 50% glycerol, pH 7.2. No preservative is contained.
Research Area Cell Cycle
Restrictions For Research Use Only

Publications:  Cyclin D3 (Human) antibody

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Publications Bartkova, Zemanova, Bartek:
"Abundance and subcellular localisation of cyclin D3 in human tumours."
In: International journal of cancer. Journal international du cancer , Vol. 65, Issue 3, pp. 323-7, 1996/01 (PubMed)

Depoortere, Van Keymeulen, Lukas et al.:
"A requirement for cyclin D3-cyclin-dependent kinase (cdk)-4 assembly in the cyclic adenosine monophosphate-dependent proliferation of thyrocytes."
In: The Journal of cell biology , Vol. 140, Issue 6, pp. 1427-39, 1998/01 (PubMed)

Depoortere, Pirson, Bartek et al.:
"Transforming growth factor beta(1) selectively inhibits the cyclic AMP-dependent proliferation of primary thyroid epithelial cells by preventing the association of cyclin D3-cdk4 with nuclear p27(kip1)."
In: Molecular biology of the cell , Vol. 11, Issue 3, pp. 1061-76, 2000/01 (PubMed)

Images:  Cyclin D3 (Human) antibody

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Western blot analysis of Cyclin D3 expression in MCF7 cells (A), Rat-1 cells (B-1) and NIH/3T3 cells (B-2) using ABIN131548.

Image for Cyclin D3 (Human) antibody (1)

Immunohistochemical detection of Cyclin D3 on paraffin embedded section of human tonsil with ABIN131548. Clear nuclear staining was shown in germinal center cells of human tonsil.

Image for Cyclin D3 (Human) antibody (2)

Immunoprecipitation of Cyclin D3 from MCF7 cells with normal mouse IgG (1) or ABIN131548 (2). After immunoprecipitated with the antibody, immunocomplex was resolved on SDS-PAGE and immunoblotted with ABIN131548.

Image for Cyclin D3 (Human) antibody (3)

External Information:  Cyclin D3 (Human) antibody

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Google Scholar Find more references for clone DCS-22 on Google Scholar™
EMBL Harvester Search human, mouse or rat genome for antigen Cyclin D3 (Bioinformatic Harvester (EMBL))