Cytochrome c antibody

Overview


	Antigen:	Cytochrome c
	Antibody Type:	Monoclonal
	Application:	Western Blotting (WB)
	Reactivity:	Most Species
	Host:	Mouse
	Quantity:	50 µl (5 WB) (Product variants)
	Price:	141,39 $ (Plus shipping costs and VAT, Invoice in EUR)
	Order number:	ABIN179787
	Availability:	Ships within 7 to 10 days

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Additional Information: Cytochrome c antibody

Antigen	Cytochrome c
Immunogen	Horse cyt c-OVA
Reactivity	Most Species
Antibody Type	Monoclonal
Format	Purified
Isotype	Mouse IgG2a, kappa »Matching secondary antibodies
Description	Cytochrome c is a 15 kD protein found in the mitochondrial intermembrane space with a heme-binding domain. Cytochrome c is a component of the electron transport chain, the heme group transfers electrons from cytochrome b-c1 complex to cytochrome oxidase complex. Cytochrome c initiates apoptosis by release to cytoplasm and binding Apaf-1 which activates procaspase 9. Cytochrome c interacts with the cytochrome b-c1 complex, cytochrome oxidase complex, heme, Apaf-1, and Caspase 9 proteins. The 7H8.2C12 monoclonal antibody recognizes cytochrome-c from most species and has been shown to be useful for Western blotting. Distribution: Mitochondrial intermembrane space Function: Component of electron transport chain, heme group transfers electrons from cytochrome b-c1 complex to cytochrome oxidase complex. Initiates apoptosis by release to cytoplasm and binding Apaf-1 which activates procaspase 9 Synonyms: Cyt c Structure: Heme binding domain, 15 kD
Clone	7H8-2C12
Host	Mouse
Specificity	Cytochrome b-c1 complex, cytochrome oxidase complex, heme, Apaf-1, Casp9.

Application Details: Cytochrome c antibody

Application	Western Blotting (WB)
Application Notes	Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 µl antibody per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
Purification	The antibody was purified by protein G affinity chromatography
Buffer	This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide, 1% BSA (origin USA) and 50% glycerol.
Storage	Upon receipt, store undiluted at -20° C.
Research Area	Metabolism
Restrictions	For Research Use only

Publications: Cytochrome c antibody

Publications

Ferguson, Marietta, Van Den Berg:
"UV-induced apoptosis is mediated independent of caspase-9 in MCF-7 cells: a model for cytochrome c resistance."
In: The Journal of biological chemistry , Vol. 278, Issue 46, pp. 45793-800, 2003/01 (PubMed)

Li, Nijhawan, Budihardjo et al.:
"Cytochrome c and dATP-dependent formation of Apaf-1/caspase-9 complex initiates an apoptotic protease cascade."
In: Cell , Vol. 91, Issue 4, pp. 479-89, 1997/01 (PubMed)

Liu, Kim, Yang et al.:
"Induction of apoptotic program in cell-free extracts: requirement for dATP and cytochrome c."
In: Cell , Vol. 86, Issue 1, pp. 147-57, 1996/01 (PubMed)

Zhang, Gerstein:
"The human genome has 49 cytochrome c pseudogenes, including a relic of a primordial gene that still functions in mouse."
In: Gene , Vol. 312, pp. 61-72, 2003/01 (PubMed)

Jemmerson, Johnson, Burrell et al.:
"A monoclonal antibody specific for a cytochrome c T cell stimulatory peptide inhibits T cell responses and affects the way the peptide associates with antigen-presenting cells."
In: European journal of immunology , Vol. 21, Issue 1, pp. 143-51, 1991/01 (PubMed)

Images: Cytochrome c antibody

Hela cell extract was resolved by electrophoresis, transferred to nitrocellulose, and probed with anti-cytochrome C (clone 7H8.2C12) antibody. Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and a chemiluminescence detection system.

Image for Cytochrome c antibody (1)

Product variants

Cytochrome c antibody (Format: Purified, Quantity: 200 µl (20 WB))

External Information: Cytochrome c antibody

Google Scholar	Find more references for clone 7H8-2C12 on Google Scholar™
EMBL Harvester	Search human, mouse or rat genome for antigen Cytochrome c (Bioinformatic Harvester (EMBL))