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Product details for  Cytokeratin 10 (Human) antibody
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Cytokeratin 10 (Human) antibody

Overview

Antigen: Cytokeratin 10
Antibody Type: Monoclonal
Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
Reactivity: Dog (Canine), Cat (Feline), Human
Host: Mouse
Quantity: 1 ml (Product variants)
Price: 367,06 $   (Plus shipping costs and VAT, Invoice in EUR)
Order number: ABIN114446
Availability: 3 to 10 days until shipment
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Additional Information:  Cytokeratin 10 (Human) antibody

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Antigen Cytokeratin 10
Immunogen Cytoskeletal preparation from human epidermis.
Reactivity Dog (Canine), Cat (Feline), Human
Antibody Type Monoclonal
Format Supernatant
Isotype IgG1 »Matching secondary antibodies
Clone DE-K10
Host Mouse
Specificity This antibody specifically recognizes the 56.6 kD cytokeratin 10 polypeptide in squamous cells and carcinomas from e.g. epidermis, ling, bladder, cervix, oesophagus. Reacts with human and most mammals (species tested so far).

Application Details:  Cytokeratin 10 (Human) antibody

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Application Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
Application Notes Immunohistochemistry on frozen and paraffin embedded sections (1:5-1:10) using an ABC method. We suggest an incubation period of 1 h at room temperature. Protease digestion: Trypsin. Western blotting. Recommended positive control: squamous cell carcinoma, epidermis. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Buffer This antibody is supplied as tissue culture supernatant . Dilute in 0.15 M PBS with 1% BSA and 0.1% Na-Azide.
Storage Store the undiluted antibody at 2-8°C for up two weeks or aliquot and store at -20°C for longer. Shelf life: one year from despatch.
Research Area Extracellular Matrix
Restrictions For Research Use only

Publications:  Cytokeratin 10 (Human) antibody

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Publications Ivanyi, Ansink, Groeneveld et al.:
"New monoclonal antibodies recognizing epidermal differentiation-associated keratins in formalin-fixed, paraffin-embedded tissue. Keratin 10 expression in carcinoma of the vulva."
In: The Journal of pathology , Vol. 159, Issue 1, pp. 7-12, 1989/01 (PubMed)

Ivanyi, Groeneveld, Van Doornewaard et al.:
"Keratin subtypes in carcinomas of the uterine cervix: implications for histogenesis and differential diagnosis."
In: Cancer research , Vol. 50, Issue 16, pp. 5143-52, 1990/01 (PubMed)

Ivanyi, Ansink, Mooi et al.:
"Absence of differentiation-related expression of keratin 10 in early stages of vulvar squamous carcinoma."
In: Differentiation; research in biological diversity , Vol. 42, Issue 2, pp. 124-9, 1990/01 (PubMed)

External Information:  Cytokeratin 10 (Human) antibody

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Google Scholar Find more references for clone DE-K10 on Google Scholar™
EMBL Harvester Search human, mouse or rat genome for antigen Cytokeratin 10 (Bioinformatic Harvester (EMBL))