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Product details for Cytokeratin 18 (Human) antibody
Cytokeratin 18 (Human) antibody
Overview
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| Antigen: | Cytokeratin 18 |
| Antibody Type: | Monoclonal |
| Application: | Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) |
| Reactivity: | Human |
| Host: | Mouse |
| Quantity: | |
| Price: | 445,14 $ (Plus shipping costs and VAT, Invoice in EUR) |
| Order number: | ABIN98166 |
| Availability: | 7 days until shipment |
Additional Information: Cytokeratin 18 (Human) antibody
| Antigen | Cytokeratin 18 |
| Immunogen | BALB/C mice were injected with PMC-42 human breast carcinoma cell line. |
| Reactivity | Human |
| Antibody Type | Monoclonal |
| Format | Supernatant |
| Isotype | IgG1 »Matching secondary antibodies |
| Clone | DC10 |
| Host | Mouse |
| Specificity | This antibody is specific to human cytokeratin 18 of 45 kD. Cytokeratin 18 belongs to a family of acidic type A and exists along with cytokeratin 8 in most simple ductal and glandular epithelia. This antibody does not reacts with squamous epithelium. It reacts with benign and malignant epithelial lesions as well as majority of adenocarcinomas and basal cell carcinomas. |
Application Details: Cytokeratin 18 (Human) antibody
| Application | Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) |
| Application Notes | Titer/Working Dilution: This antibody may be diluted to a titer of 1:25-1:75 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Skin. Cellular localization: Cytoplasmic Other Applications: Western Blotting (1:50 to 1:100) |
| Buffer | This antibody is supplied as purified immunoglobulin fraction containing sodium azide as preservative. |
| Storage | Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label. |
| Research Area | Extracellular Matrix |
| Restrictions | For Research Use only |
Images: Cytokeratin 18 (Human) antibody
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Formalin fixed paraffin embedded human breast carcinoma stained with Cytokeratin 18 antibody |
External Information: Cytokeratin 18 (Human) antibody
| Google Scholar | Find more references for clone DC10 on Google Scholar™ |
| EMBL Harvester | Search human, mouse or rat genome for antigen Cytokeratin 18 (Bioinformatic Harvester (EMBL)) |
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