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Cytokeratin (Human) antibody
Overview
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| Antigen: | Cytokeratin |
| Antibody Type: | Monoclonal |
| Application: | Immunohistochemistry (IHC), ELISA, Western Blotting (WB), Immunoprecipitation (IP) |
| Reactivity: | Human, Mouse (Murine), Rat, Sheep (Ovine) |
| Host: | Mouse |
| Quantity: | |
| Price: | 338,33 $ (Plus shipping costs and VAT, Invoice in EUR) |
| Order number: | ABIN129508 |
| Availability: | 7 days until shipment |
Additional Information: Cytokeratin (Human) antibody
| Antigen | Cytokeratin |
| Immunogen | . |
| Reactivity | Human, Mouse (Murine), Rat, Sheep (Ovine) |
| Antibody Type | Monoclonal |
| Format | Liquid (sterile filtered) |
| Clone | C11 |
| Host | Mouse |
Application Details: Cytokeratin (Human) antibody
| Application | Immunohistochemistry (IHC), ELISA, Western Blotting (WB), Immunoprecipitation (IP) |
| Concentration | 1.3 mg/ml (A280 absorbance) |
| Purity | 0.02 M Potassium Phosphate, 0.5 M NaCl, pH 7.2Stabilizer: None Preservative: 0.01% (w/v) Sodium Azide |
| Purification | This protein A purified mouse monoclonal antibody reacts specifically with keratins from humantissues and derived cell lines. This antibody reacts with keratin (56 kDa), keratin 17 (46 kDa), keratin 18 (45 kDa) andkeratin 19 (40 kDa) derived from humans. Cross reactivity with keratins from other sources has not been determined.No reaction is expected against other filament proteins including vimentin, desmin and neurofilament protein. |
| Storage | Store vial at -20° Cprior to opening. Aliquot contents andfreeze at -20° C or below for extendedstorage. Avoid cycles of freezing andthawing. Centrifuge product if notcompletely clear after standing at roomtemperature. This product is stable forseveral weeks at 4° C as an undilutedliquid. Dilute only prior to immediate use.Expiration date is one (1) year from date ofopening. |
| Research Area | Extracellular Matrix |
| Restrictions | For Research Use only |
Images: Cytokeratin (Human) antibody
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Western blot using antibodies-online Immunochemical's Mouse Mab-anti-Keratin antibody. This antibody recognizes a single 56 kDa band corresponding to human keratin as confirmed by the position of molecular weight markers (not shown). Approximatley 100 ng of keratin from human epidermis (Sigma p/n K0253) was applied under reducing conditions to a pre-cast 4-20% iGel from Gradipore Inc. A 1:400 dilution of Mab anti-Keratin was used for 2h followed by detection using a 1:5,000 dilution of IRDyeTM800 conjugated Goat-a-Mouse IgG [H&L] (610-132-121) and visualization using the Odyssey
Immunofluorescence microscopy using antibodies-online Immunochemical's Mouse Mab-anti-Keratin antibody. Confocal slices of HeLa cells are between 0.5 and 0.6 uM where the image is taken near the bottom of the cell. Use FITC a 1:2,000 dilution of FITC conjugated Goat-a- Mouse IgG [H&L] (ABIN101765) for detection. |
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