D4-GDI (Human) antibody

概述


	抗原:	D4-GDI (GDP dissociation inhibitor) (cleaved form)
	抗体类型:	单克隆抗体
	应用范围:	Flow Cytometry (FACS), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
	目标物种:	Human, Mouse (Murine)
	抗体来源:	Mouse
	规格:	0.1 mg (0.5 mg/ml)
	价格:	2.850,19 ¥ (加上运费和增值税, Invoice in EUR)
	货号:	ABIN121226
	发货时间:	Ships in 3 to 10 days

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详细信息: D4-GDI (Human) antibody

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抗原	D4-GDI (GDP dissociation inhibitor) (cleaved form)
免疫原	Synthetic peptide corresponding to the region of the Fas-induced cleavage site of human D4-GDI
目标物种	Human, Mouse (Murine)
抗体类型	单克隆抗体
状态	Purified
抗体同种型	IgG1 »相配的二抗
克隆	97A1015
抗体来源	Mouse
特异性	This antibody recognizes D4-GDI from human and mouse. D4-GDI (GDP dissociation inhibitor) is a negative regulator of the ras-related Rho family of GTPases. Since the Rho GTPases promote cytoskeletal and membrane changes associated with apoptotic cell death, the removal of the D4-GDI block through its cleavage is important for inducing apoptosis. Caspase-3 cleaves the 28 kD mature form of D4-GDI to give a 23 kD and 5 kD size fragment. The 23 kD fragment then translocates to the nucleus. The mechanisms involving cleavage of D4-GDI with apoptosis are not presently known. Activation of the Jun N-terminal kinase, a regulator of apoptosis, may be one of the mechanisms.

使用细节: D4-GDI (Human) antibody

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应用范围	Flow Cytometry (FACS), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
应用备注	Western blotting (2 ug/ml, In anti-Fas treated Jurkat cells, a ~23 kDa band should be observed). Flow Cytometry (intracellular, 0.5 – 1 ug for 106 cells). Immunoprecipitation (1-2 ug/ml). Immunohisto-chemistry (2 ug/ml). Other applications not tested. Optimal dilutions of this antibody are dependent on conditions and should be determined by the user.
浓度	0.5 mg/ml
缓冲液	This antibody is supplied as liquid, Protein G purified IgG fraction in PBS with 0.02% sodium azide as preservative. Western blot analysis: HL60 cells were treated for different time periods with 10 ng/ml of anti-Fas mAb (Clone DX2). Harvested cells were
贮藏	Store the antibody at 4-8°C for one month or at –20°C for longer. Avoid repeated freezing and tha wing. Shelf life: one year from despatch.
限制	Research only

图像: D4-GDI (Human) antibody

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Western blot analysis: HL60 cells were treated for different time periods with 10 ng/ml of anti-Fas mAb (Clone DX2). Harvested cells were lysed and resolved by SDS-PAGE and transferred onto membrane. D4-GDI cleavage was detected by ABIN121226.

图像 D4-GDI (Human) antibody (1)

外部连接: D4-GDI (Human) antibody

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Google Scholar	克隆 97A1015
EMBL Harvester	在human, mouse 或 rat 基因组里搜索抗原D4-GDI (GDP dissociation inhibitor) (cleaved form) (Bioinformatic Harvester (EMBL))