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Détail du produit pour D4-GDI (Human) antibody
D4-GDI (Human) antibody
Aperçu
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| Antigène: | D4-GDI (GDP dissociation inhibitor) (cleaved form) |
| Type anticorps: | Monoclonal |
| Application: | Flow Cytometry (FACS), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB) |
| Reactivité: | Human, Mouse (Murine) |
| Hôte: | Mouse |
| Quantité: | |
| Prix: | 310,00 € (Plus frais de livraison et TVA) |
| Numéro de commande: | ABIN121226 |
| Disponibilité: | Ships in 3 to 10 days |
Description du produit: D4-GDI (Human) antibody
| Antigène | D4-GDI (GDP dissociation inhibitor) (cleaved form) |
| Immunogène | Synthetic peptide corresponding to the region of the Fas-induced cleavage site of human D4-GDI |
| Reactivité | Human, Mouse (Murine) |
| Type anticorps | Monoclonal |
| Format | Purified |
| Isotype | IgG1 »Anticorps secondaires correspondants |
| Clone | 97A1015 |
| Hôte | Mouse |
| Specificité | This antibody recognizes D4-GDI from human and mouse. D4-GDI (GDP dissociation inhibitor) is a negative regulator of the ras-related Rho family of GTPases. Since the Rho GTPases promote cytoskeletal and membrane changes associated with apoptotic cell death, the removal of the D4-GDI block through its cleavage is important for inducing apoptosis. Caspase-3 cleaves the 28 kD mature form of D4-GDI to give a 23 kD and 5 kD size fragment. The 23 kD fragment then translocates to the nucleus. The mechanisms involving cleavage of D4-GDI with apoptosis are not presently known. Activation of the Jun N-terminal kinase, a regulator of apoptosis, may be one of the mechanisms. |
Applications: D4-GDI (Human) antibody
| Application | Flow Cytometry (FACS), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB) |
| Indications d'application | Western blotting (2 ug/ml, In anti-Fas treated Jurkat cells, a ~23 kDa band should be observed). Flow Cytometry (intracellular, 0.5 – 1 ug for 106 cells). Immunoprecipitation (1-2 ug/ml). Immunohisto-chemistry (2 ug/ml). Other applications not tested. Optimal dilutions of this antibody are dependent on conditions and should be determined by the user. |
| Concentration | 0.5 mg/ml |
| Buffer | This antibody is supplied as liquid, Protein G purified IgG fraction in PBS with 0.02% sodium azide as preservative. Western blot analysis: HL60 cells were treated for different time periods with 10 ng/ml of anti-Fas mAb (Clone DX2). Harvested cells were |
| Stock | Store the antibody at 4-8°C for one month or at –20°C for longer. Avoid repeated freezing and tha wing. Shelf life: one year from despatch. |
| Restrictions | Research only |
Images: D4-GDI (Human) antibody
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Western blot analysis: HL60 cells were treated for different time periods with 10 ng/ml of anti-Fas mAb (Clone DX2). Harvested cells were lysed and resolved by SDS-PAGE and transferred onto membrane. D4-GDI cleavage was detected by ABIN121226. |
Informations supplémentaires: D4-GDI (Human) antibody
| Google Scholar | Trouver des références supplémentaires pour clone 97A1015 sur Google Scholar™ |
| EMBL Harvester | Recherche humain, souris ou rat génome pour antigène D4-GDI (GDP dissociation inhibitor) (cleaved form) (Bioinformatic Harvester (EMBL)) |
