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Détail du produit pour D52L2 (Human) antibody
D52L2 (Human) antibody
Aperçu
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| Antigène: | D52L2 |
| Type anticorps: | Monoclonal |
| Application: | Enzyme Immunoassay (EIA), Western Blotting (WB) |
| Reactivité: | Human |
| Hôte: | Mouse |
| Quantité: | |
| Prix: | 280,00 € (Plus frais de livraison et TVA) |
| Numéro de commande: | ABIN121145 |
| Disponibilité: | Ships in 3 to 10 days |
Description du produit: D52L2 (Human) antibody
| Antigène | D52L2 |
| Immunogène | Recombinant GST-D52L2 protein |
| Reactivité | Human |
| Type anticorps | Monoclonal |
| Format | Purified |
| Isotype | IgG2b »Anticorps secondaires correspondants |
| Clone | D1C5 |
| Hôte | Mouse |
| Specificité | D52-like Protein(D52L2, also called TPD52L2) is a member of D52 gene family and overexpressed in human breastcarcinoma. This protein contains coiled-coil motif, which is responsible for homo- and heteromeric interaction, and may be a regulator of cell proliferation and calcium-mediated signal transduction. |
Applications: D52L2 (Human) antibody
| Application | Enzyme Immunoassay (EIA), Western Blotting (WB) |
| Indications d'application | The antibody has been tested by ELISA and by Western blot analysis to assure specificity and reactivity.Since application vary, however, each investigation should titrate the reagent to obtain optimal results. Recommended dilution range for Western blot analysis: 1:500 ~ 1:2000. Recommended starting dilution : 1:1000. Immunoblot analysis Recombinant GST-D52L2 was resolved by electrophoresis, transferred to PVDF membrane and probed with anti-D52L2(1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a DAP detection system. Arrow indicates. 80kD-- 25kD-- 20kD-- 15kD-- |
| Concentration | Lot specific |
| Buffer | This antibody is supplied as liquid, Protein G affinity purified immunoglobulin fraction in PBS buffer pH 7.4 with 0.08% sodium azide as preservative. |
| Stock | Store the antibody at 4-8°C for one month or at –20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch. |
| Restrictions | Research only |
Références: D52L2 (Human) antibody
| Références |
Byrne, Mattei, Basset: "Definition of the tumor protein D52 (TPD52) gene family through cloning of D52 homologues in human (hD53) and mouse (mD52)." In: Genomics , Vol. 35, Issue 3, pp. 523-32, 1996/01 (PubMed) Byrne, Nourse, Basset et al.: "Identification of homo- and heteromeric interactions between members of the breast carcinoma-associated D52 protein family using the yeast two-hybrid system." In: Oncogene , Vol. 16, Issue 7, pp. 873-81, 1998/01 (PubMed) Byrne, Tomasetto, Garnier et al.: "A screening method to identify genes commonly overexpressed in carcinomas and the identification of a novel complementary DNA sequence." In: Cancer research , Vol. 55, Issue 13, pp. 2896-903, 1995/01 (PubMed) Nourse, Mattei, Gunning et al.: "Cloning of a third member of the D52 gene family indicates alternative coding sequence usage in D52-like transcripts." In: Biochimica et biophysica acta , Vol. 1443, Issue 1-2, pp. 155-68, 1999/01 (PubMed) |
Images: D52L2 (Human) antibody
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Immunoblot analysis.,.Recombinant GST-D52L2 was resolved by electrophoresis, transferred to PVDF membrane and probed with anti-D52L2(1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a DAP detection system. Arrow indicates. |
Informations supplémentaires: D52L2 (Human) antibody
| Google Scholar | Trouver des références supplémentaires pour clone d1C5 sur Google Scholar™ |
| EMBL Harvester | Recherche humain, souris ou rat génome pour antigène D52L2 (Bioinformatic Harvester (EMBL)) |
