D52L2 (Human) antibody

Overview


	Antigen:	D52L2
	Antibody Type:	Monoclonal
	Application:	Enzyme Immunoassay (EIA), Western Blotting (WB)
	Reactivity:	Human
	Host:	Mouse
	Quantity:	0.1 ml (Lot specific)
	Price:	380,66 $ (Plus shipping costs and VAT, Invoice in EUR)
	Order number:	ABIN121145
	Availability:	Ships in 3 to 10 days

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Additional Information: D52L2 (Human) antibody

Antigen	D52L2
Immunogen	Recombinant GST-D52L2 protein
Reactivity	Human
Antibody Type	Monoclonal
Format	Purified
Isotype	IgG2b »Matching secondary antibodies
Clone	D1C5
Host	Mouse
Specificity	D52-like Protein(D52L2, also called TPD52L2) is a member of D52 gene family and overexpressed in human breastcarcinoma. This protein contains coiled-coil motif, which is responsible for homo- and heteromeric interaction, and may be a regulator of cell proliferation and calcium-mediated signal transduction.

Application Details: D52L2 (Human) antibody

Application	Enzyme Immunoassay (EIA), Western Blotting (WB)
Application Notes	The antibody has been tested by ELISA and by Western blot analysis to assure specificity and reactivity.Since application vary, however, each investigation should titrate the reagent to obtain optimal results. Recommended dilution range for Western blot analysis: 1:500 ~ 1:2000. Recommended starting dilution : 1:1000. Immunoblot analysis Recombinant GST-D52L2 was resolved by electrophoresis, transferred to PVDF membrane and probed with anti-D52L2(1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a DAP detection system. Arrow indicates. 80kD-- 25kD-- 20kD-- 15kD--
Concentration	Lot specific
Buffer	This antibody is supplied as liquid, Protein G affinity purified immunoglobulin fraction in PBS buffer pH 7.4 with 0.08% sodium azide as preservative.
Storage	Store the antibody at 4-8°C for one month or at –20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Restrictions	Research only

Publications: D52L2 (Human) antibody

Publications

Byrne, Mattei, Basset:
"Definition of the tumor protein D52 (TPD52) gene family through cloning of D52 homologues in human (hD53) and mouse (mD52)."
In: Genomics , Vol. 35, Issue 3, pp. 523-32, 1996/01 (PubMed)

Byrne, Nourse, Basset et al.:
"Identification of homo- and heteromeric interactions between members of the breast carcinoma-associated D52 protein family using the yeast two-hybrid system."
In: Oncogene , Vol. 16, Issue 7, pp. 873-81, 1998/01 (PubMed)

Byrne, Tomasetto, Garnier et al.:
"A screening method to identify genes commonly overexpressed in carcinomas and the identification of a novel complementary DNA sequence."
In: Cancer research , Vol. 55, Issue 13, pp. 2896-903, 1995/01 (PubMed)

Nourse, Mattei, Gunning et al.:
"Cloning of a third member of the D52 gene family indicates alternative coding sequence usage in D52-like transcripts."
In: Biochimica et biophysica acta , Vol. 1443, Issue 1-2, pp. 155-68, 1999/01 (PubMed)

Images: D52L2 (Human) antibody

Immunoblot analysis.,.Recombinant GST-D52L2 was resolved by electrophoresis, transferred to PVDF membrane and probed with anti-D52L2(1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a DAP detection system. Arrow indicates.

Image for D52L2 (Human) antibody (1)

External Information: D52L2 (Human) antibody

Google Scholar	Find more references for clone d1C5 on Google Scholar™
EMBL Harvester	Search human, mouse or rat genome for antigen D52L2 (Bioinformatic Harvester (EMBL))