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产品详细信息： DAP Kinase 1 (phospho S308) (Human) antibody
DAP Kinase 1 (phospho S308) (Human) antibody
概述
| 抗原: | DAP Kinase 1 (phospho S308) |
| 抗体类型: | 单克隆抗体 |
| 应用范围: | Dot Blot (Dot), ELISA, Immunoprecipitation (IP) |
| 目标物种: | Human |
| 抗体来源: | Mouse |
| 规格: | |
| 价格: | 2.491,62 ¥ (加上运费和增值税, Invoice in EUR) |
| 货号: | ABIN146423 |
| 发货时间: | 7 to 10 days until shipment |
详细信息: DAP Kinase 1 (phospho S308) (Human) antibody
| 抗原 | DAP Kinase 1 (phospho S308) |
| 免疫原 | Synthetic peptide, corresponding to amino acids 303-312 (pSER308) of Human DAP Kinase 1. |
| 目标物种 | Human |
| 抗体类型 | 单克隆抗体 |
| 状态 | Liquid |
| 抗体同种型 | IgG1 »相配的二抗 |
| 描述 | Various apoptotic signals use Death Associated Protein Kinase (DAPK) as a downstream effector in different cell types. DAPK is a positive mediator of apoptosis and is widely expressed in many tissues of embryonic and adult origin. DAPK is a Ca2+/calmodulin dependent Ser/Thr kinase that associates with microfilaments. The protein is composed of a multidomain structure. It has a subdomain typical of serine/threonine kinases, a Ca2+/calmodulin regulatory domain, eight ankyrin repeats followed by two P-loop motifs and a typical death domain module. It contains two auto-inhibitory domains one of them Ca2+/calmodulin dependent. In the absence of this latter domain, DAPK is constitutively active. DAPK activity is also regulated by phosphorylation. DAPK was found to be negatively regulated by autophosphorylation at Ser308 which is in the calmodulin regulatory domain. This autophosphorylation, which occurs in cells at the basal state, lowers the affinity of DAPK for calmodulin and thus the k |
| 克隆 | DKPS308 |
| 抗体来源 | Mouse |
使用细节: DAP Kinase 1 (phospho S308) (Human) antibody
| 应用范围 | Dot Blot (Dot), ELISA, Immunoprecipitation (IP) |
| 应用备注 | Dot: Use at a concentration of 1-2 ug/ml, Determined by immunoblotting using 293T (human embryonal kidney) cells transfected with DAPKinase expression vector. ELISA: Use at an assay dependent dilution. IP: Use at an assay dependent dilution. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user. |
| 浓度 | 2 mg/ml |
| 纯度 | Immunogen affinity purified |
| 缓冲液 | Preservative: 15mM Sodium AzideConstituents: 0.01mM PBS. pH 7.4 |
| 限制 | For research use only |
外部连接: DAP Kinase 1 (phospho S308) (Human) antibody
| Google Scholar | 克隆 DKPS308 |
| EMBL Harvester | 在human, mouse 或 rat 基因组里搜索抗原DAP Kinase 1 (phospho S308) (Bioinformatic Harvester (EMBL)) |
