DAP3 (Human) antibody

Overview


	Antigen:	DAP3
	Antibody Type:	Monoclonal
	Application:	Western Blotting (WB)
	Reactivity:	Human, Mouse (Murine)
	Host:	Mouse
	Quantity:	0.1 mg (0.5 mg/ml)
	Price:	407,85 $ (Plus shipping costs and VAT, Invoice in EUR)
	Order number:	ABIN121228
	Availability:	Ships in 3 to 10 days

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Additional Information: DAP3 (Human) antibody

Antigen	DAP3
Immunogen	The antibody was raised against a synthetic peptide corresponding to amino acids 51-66 of human DAP-3 protein. This sequence is 75% homologous in mouse and rat.
Reactivity	Human, Mouse (Murine)
Antibody Type	Monoclonal
Format	Purified
Isotype	IgG »Matching secondary antibodies
Clone	42C617
Host	Mouse
Specificity	Apoptosis, or programmed cell death, occurs during normal cellular differentiation and development of multicellular organisms. Recently, five novel apoptotic genes (named DAP genes for: Death Associated Proteins) have been identified that code for proteins, which display a diverse spectrum of biochemical activities. These include a novel type of calcium/calmodulin-regulated kinase which carries ankyrin repeats and a death domain (DAP-kinase), a nucleotide-binding protein (DAP-3), a small proline-rich cytoplasmic protein (DAP-1), and a novel homolog of the eIF4G translation initiation factor (DAP-5) (1). DAP-3 has an ATP/GTP binding motive (P-loop) and has been isolated from human and mouse, and named as hDAP-3 and mDAP-3 (Death Associated Protein-3), respectively (2-3). Sequence analysis revealed an 82% amino acid identity between human and mouse protein. In situ hybridization and northern blot analysis showed an abundant mRNA expression with a pronounced expression in highly proliferative epithelial compartments. Overexpression of DAP-3 leads to cytochrome c release and induction of cell death. DAP-3 mediated apoptosis was shown to depend on a functional P-loop. mDAP-3 is localized to the mitochondrial matrix and in contrast to cytochrome c, retained its mitochondrial localization during apoptosis induction (3). Reacts with Mouse, Human. Predicted react: rat.

Application Details: DAP3 (Human) antibody

Application	Western Blotting (WB)
Application Notes	Western blot analysis (1.0 - 2.0 ug/ml, positive control: Jurkat). In Jurkat, a 45 kDa band is observed. Other applications not tested. Optimal dilutions of this antibody are dependent on conditions and should be determined by the user.
Concentration	0.5 mg/ml
Buffer	This antibody is supplied as liquid IgG fraction, purified on Protein G, in PBS containing 0.2% gelatine as stabilizer and 0.05% sodium azide as preservative.
Storage	Store the antibody at 4°C for one month or at –20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Restrictions	Research only

Publications: DAP3 (Human) antibody

Publications

Bennouna, Bliss, Curiel et al.:
In: (PubMed)

Kissil, Deiss, Bayewitch et al.:
"Isolation of DAP3, a novel mediator of interferon-gamma-induced cell death."
In: The Journal of biological chemistry , Vol. 270, Issue 46, pp. 27932-6, 1996/01 (PubMed)

Kimchi:
"DAP kinase and DAP-3: novel positive mediators of apoptosis."
In: Annals of the rheumatic diseases , Vol. 58 Suppl 1, pp. I14-9, 2000/01 (PubMed)

Images: DAP3 (Human) antibody

Western blot analysis of 10 ug of total cell lysate from Jurkat cells with ABIN121228 at 1 ug/ml.

Image for DAP3 (Human) antibody (1)

External Information: DAP3 (Human) antibody

Google Scholar	Find more references for clone 42C617 on Google Scholar™
EMBL Harvester	Search human, mouse or rat genome for antigen DAP3 (Bioinformatic Harvester (EMBL))