DAP3 (Human) antibody

Overview


	Antigen:	DAP3
	Antibody Type:	Polyclonal
	Application:	Western Blotting (WB)
	Reactivity:	Mouse (Murine), Human, Rat
	Host:	Rabbit
	Quantity:	0.1 mg (0.5 mg/ml) (Product variants)
	Price:	380,66 $ (Plus shipping costs and VAT, Invoice in EUR)
	Order number:	ABIN123324
	Availability:	Ships in 3 to 10 days

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Additional Information: DAP3 (Human) antibody

Antigen	DAP3
Immunogen	The antibody was raised against two synthetic peptides corresponding to amino acids 44-52 and 358-371 of mouse DAP-3 protein.
Reactivity	Mouse (Murine), Human, Rat
Antibody Type	Polyclonal
Format	Purified
Host	Rabbit
Specificity	Apoptosis, or programmed cell death, occurs during normal cellular differentiation and development of multicellular organisms. Recently, five novel apoptotic genes (named DAP genes for: Death Associated Proteins) have been identified that code for proteins, which display a diverse spectrum of biochemical activities. These include a novel type of calcium/calmodulin-regulated kinase which carries ankyrin repeats and a death domain (DAP-kinase), a nucleotide-binding protein (DAP-3), a small proline-rich cytoplasmic protein (DAP-1), and a novel homolog of the eIF4G translation initiation factor (DAP-5) (4). DAP-3 has an ATP/GTP binding motive (P-loop) and has been isolated from human and mouse, and named as hDAP-3 and mDAP-3 (Death Associated Protein-3), respectively (2-3). Sequence analysis revealed an 82% amino acid identity between human and mouse protein. In situ hybridization and northern blot analysis showed an abundant mRNA expression with a pronounced expression in highly proliferative epithelial compartments. Overexpression of DAP-3 leads to cytochrome c release and induction of cell death. DAP-3 mediated apoptosis was shown to depend on a functional P-loop. mDAP-3 is localized to the mitochondrial matrix and in contrast to cytochrome c, retained its mitochondrial localization during apoptosis induction (2). Reacts with mouse and human.

Application Details: DAP3 (Human) antibody

Application	Western Blotting (WB)
Application Notes	Western blot: 1-2 ug/ml. Recommended positive control: Jurkat. In Jurkat, a 45 kDa band is observed. Other applications not tested. Optimal dilutions of this antibody are dependent on conditions and should be determined by the user.
Concentration	0.5 mg/ml
Buffer	This antibody is supplied as liquid, Protein G affinity purified IgG in PBS, pH 7.2, with 0.2% gelatine and 0.05% sodium azide as preservative.
Storage	Store the antibody at 4°C for one month or at –20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Restrictions	Research only

Publications: DAP3 (Human) antibody

Publications

Kissil, Deiss, Bayewitch et al.:
"Isolation of DAP3, a novel mediator of interferon-gamma-induced cell death."
In: The Journal of biological chemistry , Vol. 270, Issue 46, pp. 27932-6, 1996/01 (PubMed)

Kimchi:
"DAP kinase and DAP-3: novel positive mediators of apoptosis."
In: Annals of the rheumatic diseases , Vol. 58 Suppl 1, pp. I14-9, 2000/01 (PubMed)

Levy-Strumpf, Kimchi:
"Death associated proteins (DAPs): from gene identification to the analysis of their apoptotic and tumor suppressive functions."
In: Oncogene , Vol. 17, Issue 25, pp. 3331-40, 1999/01 (PubMed)

Images: DAP3 (Human) antibody

Western blot analysis of 10 ug of total cell lysate from Jurkat cells with ABIN123324at 1 ug/ml.

Image for DAP3 (Human) antibody (1)

Product variants

DAP3 (Human) antibody (Format: Aff - Purified, Quantity: 0.1 mg)

External Information: DAP3 (Human) antibody

Google Scholar	Find more references for antigen DAP3 on Google Scholar™
EMBL Harvester	Search human, mouse or rat genome for antigen DAP3 (Bioinformatic Harvester (EMBL))