DAP5 (Human) antibody

Overview


	Antigen:	DAP5
	Antibody Type:	Monoclonal
	Application:	Western Blotting (WB)
	Reactivity:	Human, Mouse (Murine)
	Host:	Mouse
	Quantity:	0.1 mg (0.5 mg/ml)
	Price:	407,85 $ (Plus shipping costs and VAT, Invoice in EUR)
	Order number:	ABIN121229
	Availability:	Ships in 3 to 10 days

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Additional Information: DAP5 (Human) antibody

Antigen	DAP5
Immunogen	The antibody was raised against two synthetic peptides corresponding to amino acids 796-812 of human DAP-5 protein.
Reactivity	Human, Mouse (Murine)
Antibody Type	Monoclonal
Format	Purified
Isotype	IgG1 »Matching secondary antibodies
Clone	39C534
Host	Mouse
Specificity	Apoptosis, or programmed cell death, occurs during normal cellular differentiation and development of multicellular organisms. Recently, five novel apoptotic genes (named DAP genes for: Death Associated Proteins) have been identified that code for proteins, which display a diverse spectrum of biochemical activities. These include a novel type of calcium/calmodulin-regulated kinase, which carries ankyrin repeats and a death domain (DAP-kinase), a nucleotide-binding protein (DAP-3), a small proline-rich cytoplasmic protein (DAP-1), and a novel homolog of the eIF4G translation initiation factor (DAP-5) (1-2). DAP-5 (also named p97 and NAT1) is a ubiquitously expressed member of the translation initiation factor eIF4G family that lacks the eIF4E binding site. DAP-5 was cloned from HeLa cells using a functional approach in attempt to isolate cDNA fragments, which convey resistance to gamma interferon (IFN-g)-induced programmed cell death (3). A fragment of the cDNA when expressed as a mini-protein of 28 kDa protects the cell from IFN-g induced apoptosis. The full-length DAP-5 cDNA codes for a protein of approximate molecular weight of 97 kDa. Low level of expression protects the cells, whereas high level of expression results in cell death (3-4). Reacts with human and mouse.

Application Details: DAP5 (Human) antibody

Application	Western Blotting (WB)
Application Notes	Western blot analysis (1.0 - 2.0 ug/ml, positive control: Jurkat). In Jurkat, a 97 kDa band is observed. Other applications not tested. Optimal dilutions of this antibody are dependent on conditions and should be determined by the user.
Concentration	0.5 mg/ml
Buffer	This antibody is supplied as liquid IgG fraction, purified on Protein G, in PBS containing 0.2% gelatine as stabilizer and 0.05% sodium azide as preservative.
Storage	Store the antibody at 4°C for one month or at –20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Restrictions	Research only

Publications: DAP5 (Human) antibody

Publications

Wittke, Maedge, Wiedemeyer et al.:
"DAP-5 is involved in MycN/IFNgamma-induced apoptosis in human neuroblastoma cells."
In: Cancer letters , Vol. 162, Issue 2, pp. 237-43, 2001/01 (PubMed)

Bennouna, Bliss, Curiel et al.:
In: (PubMed)

Kimchi:
"DAP kinase and DAP-3: novel positive mediators of apoptosis."
In: Annals of the rheumatic diseases , Vol. 58 Suppl 1, pp. I14-9, 2000/01 (PubMed)

Levy-Strumpf, Kimchi:
"Death associated proteins (DAPs): from gene identification to the analysis of their apoptotic and tumor suppressive functions."
In: Oncogene , Vol. 17, Issue 25, pp. 3331-40, 1999/01 (PubMed)

Images: DAP5 (Human) antibody

Western blot analysis of 15 ug of total cell lysate from Jurkat cells with ABIN121229 at 1 ug/ml.

Image for DAP5 (Human) antibody (1)

External Information: DAP5 (Human) antibody

Google Scholar	Find more references for clone 39C534 on Google Scholar™
EMBL Harvester	Search human, mouse or rat genome for antigen DAP5 (Bioinformatic Harvester (EMBL))