DARPP-32 pSer75 (Human) antibody

概述

详细信息
使用细节
相关产品
外部连接

抗原	DARPP-32 pSer75
免疫原	Synthetic phosphopeptide corresponding to amino acids residues surrounding thephospho Thr75 of DARPP-32. The sequence of the immunogen is identical in humanand mouse.
目标物种	Human, Mouse (Murine)
抗体类型	多克隆抗体
状态	Aff - Purified
描述	DARPP-32, a dopamine (DA) and cAMP-regulated ~32k phosphoprotein, is associatedwith dopaminoceptive neurons bearing D-1 receptors in the basal ganglia. The proteininhibits protein phosphatase I when it is phosphorylated on Thr34. In contrast, whenDARPP-32 is phosphorylated on Thr75 the protein acts as an inhibitor of PKA. Phosphorylation of DARPP is thought to play a critical role in the regulation ofdopaminergic neurotransmission. In addition, the activity of DARPP-32 is also thoughtto play important roles in the actions of alcohol, caffeine and Prozac®.
抗体来源	Rabbit
特异性	Specific for the ~34k DARPP-32 phosphorylated at Thr75. This antibody recognizes human, mouse, and rat forms of the protein. Other specieshave not been tested.

应用范围	Western Blotting (WB)
应用备注	Western and Dot blots. Suggested working dilutions: Immunoblot - 1:1,000Immunohistochemistry - NDThe investigator should determine the optimal working dilution for a specific application.
缓冲液	0.1 ml affinity purified antibody in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per mlBSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blot.
贮藏	For long term storage –80 degrees Celcius is recommended, but shorter term storage at–20 degrees Celcius is also acceptable as aliquots may be taken without freeze/thawingdue to the presence of 50% glycerol.
限制	For Research Use only