DARPP-32 pSer75 (Human) antibody

Übersicht

Antigen	DARPP-32 pSer75
Immunogen	Synthetic phosphopeptide corresponding to amino acids residues surrounding thephospho Thr75 of DARPP-32. The sequence of the immunogen is identical in humanand mouse.
Reaktivität	Human, Mouse (Murine)
Antikörper-Typ	Polyklonal
Format	Aff - Purified
Beschreibung	DARPP-32, a dopamine (DA) and cAMP-regulated ~32k phosphoprotein, is associatedwith dopaminoceptive neurons bearing D-1 receptors in the basal ganglia. The proteininhibits protein phosphatase I when it is phosphorylated on Thr34. In contrast, whenDARPP-32 is phosphorylated on Thr75 the protein acts as an inhibitor of PKA. Phosphorylation of DARPP is thought to play a critical role in the regulation ofdopaminergic neurotransmission. In addition, the activity of DARPP-32 is also thoughtto play important roles in the actions of alcohol, caffeine and Prozac®.
Wirt	Rabbit
Spezifität	Specific for the ~34k DARPP-32 phosphorylated at Thr75. This antibody recognizes human, mouse, and rat forms of the protein. Other specieshave not been tested.

Applikation	Western Blotting (WB)
Applikationshinweise	Western and Dot blots. Suggested working dilutions: Immunoblot - 1:1,000Immunohistochemistry - NDThe investigator should determine the optimal working dilution for a specific application.
Buffer	0.1 ml affinity purified antibody in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per mlBSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blot.
Lagerung	For long term storage –80 degrees Celcius is recommended, but shorter term storage at–20 degrees Celcius is also acceptable as aliquots may be taken without freeze/thawingdue to the presence of 50% glycerol.
Beschränkungen	For Research Use only

Google Scholar	Suchen Sie in Google Scholar™ nach weiteren Artikeln für Antigen DARPP-32 pSer75
EMBL Harvester	Genom von Mensch, Maus oder Ratte nach Antigen DARPP-32 pSer75 durchsuchen (Bioinformatic Harvester (EMBL))