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Product details for D-Amino-Acid-Oxidase HRP (Pig (Porcine)) antibody
D-Amino-Acid-Oxidase HRP (Pig (Porcine)) antibody
Overview
| Antigen: | D-Amino-Acid Oxidase |
| Antibody Type: | Polyclonal |
| Application: | Enzyme Immunoassay (EIA), Western Blotting (WB) |
| Conjugate: | HRP |
| Reactivity: | Pig (Porcine) |
| Host: | Rabbit |
| Quantity: | |
| Price: | 462,23 $ (Plus shipping costs and VAT, Invoice in EUR) |
| Order number: | ABIN116618 |
| Availability: | Ships in 3 to 10 days |
Additional Information: D-Amino-Acid-Oxidase HRP (Pig (Porcine)) antibody
| Antigen | D-Amino-Acid Oxidase |
| Immunogen | D-Amino Acid Oxidase [Pig Kidney] |
| Reactivity | Pig (Porcine) |
| Antibody Type | Polyclonal |
| Host | Rabbit |
| Specificity | This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Sheep Serum as well as purified and partially purified D-Amino Acid Oxidase [Pig Kidney]. Cross reactivity against D-Amino Acid Oxidase from other tissues and species may occur but have not been specifically determined. |
Application Details: D-Amino-Acid-Oxidase HRP (Pig (Porcine)) antibody
| Application | Enzyme Immunoassay (EIA), Western Blotting (WB) |
| Application Notes | Suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays. This product has been assayed against 1.0 ug of D-Amino Acid Oxidase [Pig Kidney] in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:12,000 to 1:60,000 of the reconstitution concentration is suggested for this product. Optimal titers for other applications should be determined by the researcher. Other applications not tested. |
| Conjugate | HRP |
| Concentration | 10.0 mg/ml (by UV absorbance at 280 nm) |
| Buffer | This antibody was lyophilised from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 10 mg/ml BSA (IgG and Protease free) and 0.01% Gentamicin Sulfate as preservative. This Ig fraction is conjugated to Horseradish Peroxidase (HRP). |
| Storage | Store vial at 4°C prior to restoration. Restore with 2.0 ml of deionized water (or equivalent). For extended storage reconstitute product with 50% glycerol instead of water and then aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of restoration. |
| Research Area | Enzymes |
| Restrictions | Research only |
Publications: D-Amino-Acid-Oxidase HRP (Pig (Porcine)) antibody
| Publications |
Farr, Nakane: "Immunohistochemistry with enzyme labeled antibodies: a brief review." In: Journal of immunological methods , Vol. 47, Issue 2, pp. 129-44, 1982/01 (PubMed) |
External Information: D-Amino-Acid-Oxidase HRP (Pig (Porcine)) antibody
| Google Scholar | Find more references for antigen D-Amino-Acid Oxidase on Google Scholar™ |
| EMBL Harvester | Search human, mouse or rat genome for antigen D-Amino-Acid Oxidase (Bioinformatic Harvester (EMBL)) |
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