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Product details for DFF45/ICAD (Human) antibody
DFF45/ICAD (Human) antibody
Overview
| Antigen: | ICAD/DFF45 |
| Antibody Type: | Monoclonal |
| Application: | Western Blotting (WB) |
| Reactivity: | Human |
| Host: | Mouse |
| Quantity: | |
| Price: | 611,77 $ (Plus shipping costs and VAT, Invoice in EUR) |
| Order number: | ABIN111423 |
| Availability: | 3 to 10 days until shipment |
Additional Information: DFF45/ICAD (Human) antibody
| Antigen | ICAD/DFF45 |
| Immunogen | GST-human ICAD/DFF45 fusion protein corresponding to full length amino acid sequence (aa 1-331) |
| Reactivity | Human |
| Antibody Type | Monoclonal |
| Format | Purified |
| Isotype | IgG1 »Matching secondary antibodies |
| Clone | 6B8 |
| Host | Mouse |
| Specificity | DFF45 is the human homologue of mouse ICAD which was identified as an inhibitor of caspase-activated deoxyribonuclease (CAD). ICAD/DFF45 is a downstream regulator of DNA degradation. CAD is an apoptotic DNase with high specific activity and exists as an inactive complex with ICAD/DFF45 in living cells. Upon apoptosis, caspase 3 is activated and cleaves ICAD/DFF45, which releases CAD from ICAD/CFF45. CAD then could enter the nucleus to degrade the chromosomal DNA. ICAD/DFF45 may inhibit not only the DNase activity of CAD, but also its translocation into the nucleus. Since functional CAD can only be synthesized in the presence of ICAD/DFF45 in a cell-free system, ICAD/DFF45 seems to function as a chaperone for CAD during its synthesis. The 6B8 monoclonal antibody reacts with human ICAD/DFF45 on Western blot. Using total cell lysates from Jurkat, Raji or U937 cells, this antibody detects two bands of 45 kDa and 40 kDa corresponding to human ICAD-L and ICAD-S respectively. The 6B8 antibody does not crossreact with mouse ICAD. |
Application Details: DFF45/ICAD (Human) antibody
| Application | Western Blotting (WB) |
| Application Notes | Immunoblotting. Other applications not tested. Optimal dilutions of this antibody are dependent on conditions and should be determined by the user. |
| Buffer | This antibody is supplied lyophilized from a Protein A affinity purified immunoglobulin fraction in PBS containing 1%sucrose without preservatives. Reconstitute with 100 ul of sterile water. Bring to 18-25°C prior t use. |
| Storage | Store lyophilized product at 2-8°C. After reconstitution, aliquot and store at –20°C. Avoid multiple freeze/thaw cycles. The addition of 0.1% (w/v) sodium azide is recommended for storage of the reconstituted form for up to one month at 2-8°C. |
| Research Area | Apoptosis/Necrosis |
| Restrictions | For Research Use only |
Publications: DFF45/ICAD (Human) antibody
| Publications |
Cohen: "Caspases: the executioners of apoptosis." In: The Biochemical journal , Vol. 326 ( Pt 1), pp. 1-16, 1997/01 (PubMed) Cryns, Yuan: "Proteases to die for." In: Genes & development , Vol. 12, Issue 11, pp. 1551-70, 1998/01 (PubMed) Enari, Sakahira, Yokoyama et al.: "A caspase-activated DNase that degrades DNA during apoptosis, and its inhibitor ICAD." In: Nature , Vol. 391, Issue 6662, pp. 43-50, 1998/01 (PubMed) Liu, Zou, Slaughter et al.: "DFF, a heterodimeric protein that functions downstream of caspase-3 to trigger DNA fragmentation during apoptosis." In: Cell , Vol. 89, Issue 2, pp. 175-84, 1997/01 (PubMed) Mitamura, Ikawa, Mizuno et al.: "Cytosolic nuclease activated by caspase-3 and inhibited by DFF-45." In: Biochemical and biophysical research communications , Vol. 243, Issue 2, pp. 480-4, 1998/01 (PubMed) Nicholson, Thornberry: "Caspases: killer proteases." In: Trends in biochemical sciences , Vol. 22, Issue 8, pp. 299-306, 1997/01 (PubMed) Sakahira, Enari, Nagata: "Cleavage of CAD inhibitor in CAD activation and DNA degradation during apoptosis." In: Nature , Vol. 391, Issue 6662, pp. 96-9, 1998/01 (PubMed) |
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