DIO2 (Human) antibody

Overview

Antigen	DIO2
Gene ID	1734 , 13371 (mouse) , 65162 (rat)
Immunogen	Peptide with sequence EVKKHQNQEDRC, from the internal region of the protein sequence according to NP_000784.2, NP_054644.1, NP_001007024.1
Reactivity	Human, Mouse (Murine), Rat
Antibody Type	Polyclonal
Format	Aff - Purified
Description	Official Gene Symbol: DIO2 Accession Number(s): NP_000784.2, NP_054644.1, NP_001007024.1. . Principal Names: DIO2, deiodinase, iodothyronine, type II , 5DII, D2, SelY, TXDI2 , thyroxine deiodinase, type II, type 2 iodothyronine deiodinase, type II iodothyronine deiodinaseN: type-II 5'deiodinase.
Host	Goat
Specificity	Expected reactivity from sequence similarity: Human, Mouse, Rat

Application Notes	Peptide ELISA: antibody detection limit dilution 1:128,000. Western Blot: Preliminary experiments gave an approx 65-70kDa band in Human Heart and Thyroid lysates after 0.05µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 305kDa according to NP_000784.2. The 65-70kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Format	Aff - Purified
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Buffer	0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Storage	Aliquot and store at -20°C. Minimize freezing and thawing.
Restrictions	For Research Use Only

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EMBL Harvester	Search human, mouse or rat genome for antigen DIO2 (Bioinformatic Harvester (EMBL))