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Product details for DNA Fragmentation Factor 45 (Human) antibody
DNA Fragmentation Factor 45 (Human) antibody
Overview
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| Antigen: | ICAD/DFF45 |
| Antibody Type: | Polyclonal |
| Application: | Immunoprecipitation (IP), Western Blotting (WB) |
| Reactivity: | Human |
| Host: | Rabbit |
| Quantity: | |
| Price: | 543,80 $ (Plus shipping costs and VAT, Invoice in EUR) |
| Order number: | ABIN123029 |
| Availability: | Ships in 3 to 10 days |
Additional Information: DNA Fragmentation Factor 45 (Human) antibody
| Antigen | ICAD/DFF45 |
| Immunogen | Synthetic Peptide: E(2) V T G D A G V P E S G E I R T L K P C(21). |
| Reactivity | Human |
| Antibody Type | Polyclonal |
| Format | Purified |
| Host | Rabbit |
| Specificity | Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. These death signals finally cause the degradation of chromosomal DNA by activated DNase. A human DNA fragmentation factor (DFF) was identified recently which was cleaved by caspase-3 during apoptosis. A mouse homologue of human DFF was identified as inhibitor of caspase activated deoxyribonuclease (ICAD). Upon cleavage of DFF/ICAD, a caspase activated deoxyribonuclease (CAD) is released and activated and eventually causes the degradation of DNA in the nuclei. Therefore, the cleavage of CAD inhibitor molecule DFF/ICAD, which causes DNase activation and DNA degradation, is the hallmark of apoptotic cell death. ABIN123029 detects DNA fragmentation factor 45 (DFF45) from human cells. This antibody detects both the cleaved and non-cleaved forms of human DFF. ABIN123029 has been successfully used in Western blot and immunoprecipitation procedures. By Western blot, this antibody detects an ~40 kDa protein representing DFF45 from HeLa cell lysate. ABIN123029 immunizing peptide corresponds to amino acid residues 2-21 from human DFF45. ABIN123029 immunizing peptide (SP5430CP) is available for use in neutralization and control experiments. |
Application Details: DNA Fragmentation Factor 45 (Human) antibody
| Application | Immunoprecipitation (IP), Western Blotting (WB) |
| Application Notes | Western Blot: 1/1000. Immunoprecipitation. Other applications not tested. Optimal dilutions of this antibody are dependent on conditions and should be determined by the user. |
| Concentration | 1.0 mg/ml |
| Buffer | This antibody is supplied as liquid, purified rabbit IgG in PBS containing 0.02% sodium azide as preservative. |
| Storage | Store the antibody at -20°C. Avoid repeated freezing and thawing. Shelf life: one year from despatch. |
| Research Area | Apoptosis/Necrosis |
| Restrictions | Research only |
Images: DNA Fragmentation Factor 45 (Human) antibody
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Figure 1 shows a Western blot of DFF45 in HeLa cell lysate using ABIN123029. |
External Information: DNA Fragmentation Factor 45 (Human) antibody
| Google Scholar | Find more references for antigen ICAD/DFF45 on Google Scholar™ |
| EMBL Harvester | Search human, mouse or rat genome for antigen ICAD/DFF45 (Bioinformatic Harvester (EMBL)) |
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