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Product details for DNA Ligase 1 (Human) antibody
DNA Ligase 1 (Human) antibody
Overview
| Antigen: | DNA Ligase 1 |
| Antibody Type: | Monoclonal |
| Application: | Western Blotting (WB), Immunoprecipitation (IP) |
| Reactivity: | Human |
| Host: | Mouse |
| Quantity: | |
| Price: | 475,82 $ (Plus shipping costs and VAT, Invoice in EUR) |
| Order number: | ABIN151048 |
| Availability: | Ships in 7 to 10 days |
Additional Information: DNA Ligase 1 (Human) antibody
| Antigen | DNA Ligase 1 |
| Immunogen | Recombinant human DNA Ligase I protein encoding amino acids 1-919 purified frombaculovirus infected Sf9 cells. Cellular Location: Nuclear |
| Reactivity | Human |
| Antibody Type | Monoclonal |
| Format | Purified |
| Isotype | IgG1 »Matching secondary antibodies |
| Description | There are four distinct ligase proteins found in mammalian cells. The activity of theseDNA ligases is required for the joining of DNA strand breaks that occur during DNAreplication, repairand recombination. DNA ligase I migrates as a 125kDaphosphoprotein and is implicated in joining Okazaki fragments during DNA synthesis. Italso has a role in the base and nucleotide excision repair pathways. |
| Clone | 10H5 |
| Host | Mouse |
| Specificity | This antibody is specific for DNA ligase 1. Human |
Application Details: DNA Ligase 1 (Human) antibody
| Application | Western Blotting (WB), Immunoprecipitation (IP) |
| Application Notes | Suggested working dilutions *Western Blot, immunoprecipitation * Investigator should determine optimal working dilutions. By Western blot, NB 100-119 recognizes DNA ligase 1 at 125 kDa. This antibody mayalso be used to immunoprecipitate active ligase. Western Blot 1-5 ug/mlImmunohistochemistry NDImmunoprecipitation 1-5 ug/ml*The investigator should determine the optimal working dilution for a specific application. * Other applications have not been tested. Positive Control:MCF-7 cells, recombinant fusion protein. |
| Buffer | 100 ug (1 mg/ml) of protein G purified antibody from hybridoma culture supernatant in10 mM PBS, pH 7.4. |
| Storage | For long-term storage aliquot and keep at -20 degrees C. Avoid repeated freeze-thawcycles. |
| Restrictions | For Research Use only |
External Information: DNA Ligase 1 (Human) antibody
| Google Scholar | Find more references for clone 10H5 on Google Scholar™ |
| EMBL Harvester | Search human, mouse or rat genome for antigen DNA Ligase 1 (Bioinformatic Harvester (EMBL)) |
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