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Product details for DNA Ligase III (Human) antibody
DNA Ligase III (Human) antibody
Overview
| Antigen: | DNA Ligase III |
| Antibody Type: | Monoclonal |
| Application: | Western Blotting (WB), Immunoprecipitation (IP) |
| Reactivity: | Human |
| Host: | Mouse |
| Quantity: | |
| Price: | 462,23 $ (Plus shipping costs and VAT, Invoice in EUR) |
| Order number: | ABIN151084 |
| Availability: | Ships in 7 to 10 days |
Additional Information: DNA Ligase III (Human) antibody
| Antigen | DNA Ligase III |
| Immunogen | Recombinant human DNA Ligase III protein encoding amino acids 1-862 purified frombaculovirus-infected Sf-9 cells. |
| Reactivity | Human |
| Antibody Type | Monoclonal |
| Format | Purified |
| Isotype | IgG1 »Matching secondary antibodies |
| Description | There are four distinct ligase proteins found in mammalian cells. The activity of theseDNA ligases is required for the joining of DNA strand breaks that occur during DNAreplication, repair and recombination. DNA Ligase III gene product migrates as a100kDa (a-form) protein in somatic tissues, is ubiquitously expressed and complexeswith XRCC-1 and a 97kDa (ß-form) protein that is found in testis. DNA Ligase III isinvolved in rejoining DNA single-strand breaks and in the base excision repair pathway. |
| Clone | 6G9 |
| Host | Mouse |
| Specificity | This antibody is specific for DNA ligase III. ABIN151084 reacts with human DNA Ligase III. |
Application Details: DNA Ligase III (Human) antibody
| Application | Western Blotting (WB), Immunoprecipitation (IP) |
| Application Notes | Suggested working dilutions *Western Blot, immunoprecipitation * Investigator should determine optimal working dilutions. Immunoblotting and ImmunoprecipitationSuggested working dilutions: *Western Blot 1-5 ug/mlImmunoprecipitation 1-5 ug/ml*The investigator should determine the optimal working dilution for a specific application. * Other applications have not been tested. Positive Control:MCF7, T24 cells and recombinant fusion protein. |
| Buffer | 100 ug (1mg/ml) of antibody purified from hybridoma cell culture supernatant by proteinG chromatography to at least 95% homogeneity as determined by SDS-PAGEchromatography. Prepared in 10mM PBS, pH 7.4. |
| Storage | Store at -20 degrees C. Avoid repeated freeze-thaw cycles. |
| Restrictions | For Research Use only |
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