DNA Ligase I antibody

Overview


	Antigen:	DNA Ligase I
	Antibody Type:	Monoclonal
	Application:	Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FACS)
	Host:	Mouse, Rat
	Quantity:	100 µg (G K0190-3: 1 mg/mL)
	Price:	327,64 $ (Plus shipping costs , $13.59 dry ice fee and VAT, Invoice in EUR)
	Order number:	ABIN131772
	Availability:	7 to 10 days until shipment

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Additional Information: DNA Ligase I antibody

Antigen	DNA Ligase I
Immunogen	This antibody was purified from hybridoma (clone 5H5) supernatant using protein A agarose. This hybridoma was established by fusion of mouse myeloma cell NSO with Balb/c mouse splenocyte immunized with the human recombinant DNA Ligase I.
Antibody Type	Monoclonal
Description	DNA ligases are ATP-dependent, nuclear enzymes that catalyze the joining of single and double-strand DNA breaks. Four distinct DNA ligases, designated DNA Ligase I-IV, have been identified in mammalian cells. The 125 kDa DNA Ligase I is a phosphoprotein responsible for joining Okazaki fragments during lagging strand DNA replication. In cooperation with replication factors A and C, DNA polymerase epsilon, and PCNA, it also plays a role in the base and nucleotide excision repair pathways. Deficiencies in DNA Ligase I result in hypersensitivity to DNA-damaging agents and aberrant DNA repair and replication.
Clone	Mouse IgG1
Host	Mouse, Rat
Specificity	This antibody reacts with DNA Ligase I (125kDa) on Western blotting.

Application Details: DNA Ligase I antibody

Application	Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FACS)
Application Notes	Western blotting: 0.2 µg/mL. Immunoprecipitation: 2µg / 200µL of cell extract from 5x10 6 cells. Immunohistochemistry: 10 µg / mL Heat treatment is necessary for paraffin embedded sections. Microwave oven: 5 minutes in Target Retrieval Solution (Dako JAPAN: code no. S1700). Immunocytochemistry: 10 µg / mL. Flow Cytometry: 10 µg / mL (final concentration)
Concentration	G K0190-3: 1 mg/mL
Buffer	100 µg IgG in 100 µL of PBS containing 50% glycerol, pH 7.2. Contains no preservatives.
Research Area	DNA/RNA
Restrictions	For Research Use Only

Publications: DNA Ligase I antibody

Publications

Bentley, Harrison, Ketchen et al.:
"DNA ligase I null mouse cells show normal DNA repair activity but altered DNA replication and reduced genome stability."
In: Journal of cell science , Vol. 115, Issue Pt 7, pp. 1551-61, 2002/01 (PubMed)

Montecucco, Rossi, Levin et al.:
"DNA ligase I is recruited to sites of DNA replication by an interaction with proliferating cell nuclear antigen: identification of a common targeting mechanism for the assembly of replication factories."
In: The EMBO journal , Vol. 17, Issue 13, pp. 3786-95, 1998/01 (PubMed)

Sun, Urrabaz, Kelly et al.:
"Enhancement of DNA ligase I level by gemcitabine in human cancer cells."
In: Clinical cancer research : an official journal of the American Association for Cancer Research , Vol. 8, Issue 4, pp. 1189-95, 2002/01 (PubMed)

Vitolo, Lidonnici, Montecucco et al.:
"A new monoclonal antibody against DNA ligase I is a suitable marker of cell proliferation in cultured cell and tissue section samples."
In: European journal of histochemistry : EJH , Vol. 49, Issue 4, pp. 349-54, 2005/01 (PubMed)

Images: DNA Ligase I antibody

Western blot analysis of D N A Ligase I expression in Jurkat cells (1), R aji cells (2), H L60 cells (3), H eLa cells (4), W R 19L cells (5) and P C 12 cells (6) using K 0190-3.

Image for DNA Ligase I antibody (1)

Immunocytochemical detection of DNA Ligase I on acetone fixed HEp-? cells with ABIN131772.

Image for DNA Ligase I antibody (2)

Immunohistochemical detection of DNA Ligase I on paraffin embedded section of a human breast cancer with ABIN131772.

Image for DNA Ligase I antibody (3)

External Information: DNA Ligase I antibody

Google Scholar	Find more references for clone Mouse IgG1 on Google Scholar™
EMBL Harvester	Search human, mouse or rat genome for antigen DNA Ligase I (Bioinformatic Harvester (EMBL))