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Product details for DNA Methyltransferase 1 (Human) antibody
DNA Methyltransferase 1 (Human) antibody
Overview
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| Antigen: | DNA Methyltransferase 1 (Dnmt1) |
| Antibody Type: | Polyclonal |
| Application: | Western Blotting (WB) |
| Reactivity: | Human |
| Host: | Rabbit |
| Quantity: | |
| Price: | 448,63 $ (Plus shipping costs and VAT, Invoice in EUR) |
| Order number: | ABIN122929 |
| Availability: | Ships in 3 to 10 days |
Additional Information: DNA Methyltransferase 1 (Human) antibody
| Antigen | DNA Methyltransferase 1 (Dnmt1) |
| Immunogen | Synthetic Peptide: A(171) K G P A K R K P Q E E S E(185). |
| Reactivity | Human |
| Antibody Type | Polyclonal |
| Format | Aff - Purified |
| Host | Rabbit |
| Specificity | DNA methyltransferases (Dnmts) methylate the 5-position of cytosine in the context of CpG dinucleotides. DNA methylation is crucial for normal embryonic development, genetic imprinting, and X-chromosome inactivation. Hypermethylation of DNA has been implicated in tumorigenesis through improper methylation of growth regulatory gene promoter regions. There exist three isoforms of Dnmts, Dnmt1, 2 and 3. Dnmt1 is believed to perform most of the maintenance and de novo methylation activities that occur in somatic cells of mammals. Studies have shown that Dnmt1 can establish repression of transcription complexes consisting of Dnmt1, histone deacetylase 2 (HDAC2), and DNA methyltransferase 1 associated protein (DMAP1). Therefore, in addition to DNA methylation maintenance, Dnmt1 is thought to directly target transcriptionally repressive chromatin to the genome during S-phase DNA replication. ABIN122929 detects DNA methyltransferase 1 (Dmnt1) from HeLa cell nuclear extracts. ABIN122929 has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~180 kDa protein representing Dnmt1 from HeLa cell nuclear extract. A doublet between 40 and 45 kDa is also seen on Western blots using this antibody. ABIN122929 immunizing peptide corresponds to amino acid residues 171-185 from human Dnmt1 protein. |
Application Details: DNA Methyltransferase 1 (Human) antibody
| Application | Western Blotting (WB) |
| Application Notes | Western Blot: 0.5 – 1.0 ug/ml. Other applications not tested. Optimal dilutions of this antibody are dependent on conditions and should be determined by the user. |
| Concentration | 1 mg/ml |
| Buffer | This antibody is supplied as liquid, epitope affinity-purified IgG in PBS containing 1 mg/ml BSA and 0.05% sodium azide as preservative. Figure 1 shows a Western blot of Dnmt1 on HeLa cell nuclear extract using ABIN122929. |
| Research Area | DNA/RNA |
| Restrictions | Research only |
Images: DNA Methyltransferase 1 (Human) antibody
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Figure 1 shows a Western blot of Dnmt1 on HeLa cell nuclear extract using ABIN122929. |
External Information: DNA Methyltransferase 1 (Human) antibody
| Google Scholar | Find more references for antigen DNA Methyltransferase 1 (Dnmt1) on Google Scholar™ |
| EMBL Harvester | Search human, mouse or rat genome for antigen DNA Methyltransferase 1 (Dnmt1) (Bioinformatic Harvester (EMBL)) |
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