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Product details for DNA Polymerase beta (Human) antibody
DNA Polymerase beta (Human) antibody
Overview
| Antigen: | DNA Polymerase beta |
| Antibody Type: | Monoclonal |
| Application: | Immunoprecipitation (IP), Western Blotting (WB) |
| Reactivity: | Human, Mouse (Murine), Rat, Bovine, Hamster |
| Host: | Mouse |
| Quantity: | |
| Price: | 382,02 $ (Plus shipping costs and VAT, Invoice in EUR) |
| Order number: | ABIN145721 |
| Availability: | 7 to 10 days until shipment |
Additional Information: DNA Polymerase beta (Human) antibody
| Antigen | DNA Polymerase beta |
| Immunogen | Full length protein (Rat). |
| Reactivity | Human, Mouse (Murine), Rat, Bovine, Hamster |
| Antibody Type | Monoclonal |
| Format | Liquid |
| Isotype | IgG1 »Matching secondary antibodies |
| Description | DNA polymerase beta comprises an amino-terminal 8 kDa domain and a carboxy terminal 31 kDa domain. The N-terminal ssDNA binding domain has a deoxyribose phosphodiesterase activity while the C-terminal domain has a nucleotidyl transferase activity. Mammalian DNA polymerase beta, a DNA repair polymerase, is constitutively expressed in cultured cells, but treatment of cells with the DNA-alkylating agents such as N-methyl-N-nitro-N-nitrosoguanidine (MNNG) or methyl methanesulphonate up-regulates beta-pol level. DNA polymerase beta fills single nucleotide gaps in DNA produced by the base excision repair pathway of mammalian cells. |
| Clone | 18S |
| Host | Mouse |
| Specificity | Shows no effect on the polymerase activity of beta-pol. |
Application Details: DNA Polymerase beta (Human) antibody
| Application | Immunoprecipitation (IP), Western Blotting (WB) |
| Application Notes | Immunoprecipitation (denatured): use at a concentration of 2ug / mg. Western Blotting: use at a concentration of 1-2ug / ml for 2hrs at RT. Not tested in other applications. Optimal dilutions / concentrations should be determined by the end user. By Western blot, this antibody detects a band of 39 kDa, which corresponds to the predicted molecular weight of DNA Polymerase beta. |
| Concentration | 0.2 mg/ml |
| Purity | Protein G purified |
| Buffer | 10mM PBS, pH7.4, 0.2%BSA, 0.09% sodium azide |
| Storage | Refrigerate at 4 C. Do not freeze. |
| Research Area | Cell Cycle, DNA/RNA, Enzymes |
| Restrictions | For research use only |
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