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Product details for DNA Polymerase e (pol e) (Hamster) antibody
DNA Polymerase e (pol e) (Hamster) antibody
Overview
| Antigen: | DNA Polymerase epsilon (pol e) |
| Antibody Type: | Monoclonal |
| Application: | Western Blotting (WB), Immunofluorescence (IF) |
| Reactivity: | Human, Mouse (Murine), Monkey, Hamster, Mink |
| Host: | Mouse |
| Quantity: | |
| Price: | 421,44 $ (Plus shipping costs and VAT, Invoice in EUR) |
| Order number: | ABIN151041 |
| Availability: | Ships in 7 to 10 days |
Additional Information: DNA Polymerase e (pol e) (Hamster) antibody
| Antigen | DNA Polymerase epsilon (pol e) |
| Immunogen | Mono S FPLC column purified HeLa cell pol epsilon. |
| Reactivity | Human, Mouse (Murine), Monkey, Hamster, Mink |
| Antibody Type | Monoclonal |
| Format | Purified |
| Isotype | IgG2a »Matching secondary antibodies |
| Description | DNA polymerase e from HeLa cells differs from yeast pol e in that HeLa pol e containstwo subunits of 220 and 55 kDa, whereas yeast pol e has one large subunit and threesmaller subunits. Previous studies of template specificity of HeLa pol e, which appearsto be required for long patch DNA repair in permeabilized cells, indicate pol e preferslong stretches of single-stranded regions in poly(dA)-oligo(dT) as template. HeLa pol aand pol d preferactivated DNA and alternating poly(dA-dT) as templates, respectively. Pol e retains itsprocessivity in the absence of proliferating cell nuclear antigen (PCNA), unlike pol d. PCNA can also interact with Pol e on linear DNA templates, even in the absence ofother auxiliary factors (replication factor C, replication factor A), and thereby stimulate itsprimer recognition and DNA synthesis. Synonyms: CHRAC 17 antibody, Chromatin Accessibility Complex 17 antibody, DNA PolymeraseEpsilon p17 Subunit antibody, DNA Polymerase Epsilon Subunit 3 antibody, 17antibody, YBL1 antibody |
| Clone | 3C5-1 |
| Host | Mouse |
| Specificity | This antibody is specific to the human DNA pol epsilon and does not recognize DNA polalpha, beta or delta. ABIN151041 can be used for detection of pol epsilon in human, mouse, monkey, mink,and hamster samples. It does not work on Drosophilia or the baculovirus host cell polepsilon, and weakly, if at all, on S. cerivisae pol epsilon. |
Application Details: DNA Polymerase e (pol e) (Hamster) antibody
| Application | Western Blotting (WB), Immunofluorescence (IF) |
| Application Notes | This antibody can be used in Western blot analysis and detects a single band of 220kDa representing the catalytic subunit of DNA Pol epsilon. NB 100-115 willimmunoprecipitate, but only in the absence of salt and even then poorly. Suggested Working Dilution: Western blot: 5 ug/ml |
| Concentration | 16 mg/ml |
| Buffer | 50 ul of Protein G purified mouse ascites at a concentration of 16 mg/ml. |
| Storage | Store at -20°C. Avoid repeated freeze-thaw cycles. |
| Restrictions | For Research Use only |
External Information: DNA Polymerase e (pol e) (Hamster) antibody
| Google Scholar | Find more references for clone 3C5-1 on Google Scholar™ |
| EMBL Harvester | Search human, mouse or rat genome for antigen DNA Polymerase epsilon (pol e) (Bioinformatic Harvester (EMBL)) |
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