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Product details for  DR5 Mouse (Murine) antibody
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DR5 Mouse (Murine) antibody

Overview

Antigen: DR5
Antibody Type: Monoclonal
Application: Flow Cytometry (FACS)
Reactivity: Mouse (Murine)
Host: Hamster
Quantity: 50 µg (Product variants)
Price: 118,28 $   (Plus shipping costs and VAT, Invoice in EUR)
Order number: ABIN157008
Availability: Ships within 7 to 10 days
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Additional Information:  DR5 Mouse (Murine) antibody

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Antigen DR5
Immunogen Mouse DR5-Ig fusion protein
Reactivity Mouse (Murine)
Antibody Type Monoclonal
Format Purified
Isotype Armenian Hamster IgG »Matching secondary antibodies
Description CD262 is a 55 kD protein also known as DR5, TRAIL-R2, Apo-2, TRICK2, and KILLER. DR5 is member 10B of the TNF receptor superfamily (TNFRSF10B), widely expressed in normal tissues and some tumor cell lines. The DR5 receptor binds to TRAIL and interacts with the cytoplasmic proteins TRADD and RIP. TRAIL binding to DR5 activates NF- kappaB and induces apoptosis. DR5 is induced by wild-type p53. The MD5 antibody is useful for flow cytometry and activation of DR5 to induce cell killing in vitro and in vivo. Distribution: Widely expressed in normal tissues and some tumor cell lines. Expression regulated by the tumor suppressor p53 Function: Binds to TRAIL, activates NF- kappaB, induces apoptosis. May be involved in the regulation of tumor metastases. Synonyms: DR5, TRAIL-R2, TNFRS10B, TRICK2, KILLER Structure: Type I transmembrane protein, TNF receptor superfamily member, homotrimer, predicted molecular weight 55 kD
Clone MD5-1
Host Hamster

Application Details:  DR5 Mouse (Murine) antibody

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Application Flow Cytometry (FACS)
Application Notes Additional reported applications (for the relevant formats) include: in vitro and in vivo induction of cytotoxicity. The LEAF(TM) purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm sterile-filtered) is recommended for functional assays (ABIN157015). Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is < 0.5 µg per 10 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance in each application.
Purification The antibody was purified by affinity chromatography.
Buffer Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Storage The antibody solution should be stored undiluted at 4 °C.
Research Area Signaling, Apoptosis/Necrosis
Restrictions For Research Use only

Publications:  DR5 Mouse (Murine) antibody

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Publications Sheridan, Marsters, Pitti et al.:
"Control of TRAIL-induced apoptosis by a family of signaling and decoy receptors."
In: Science (New York, N.Y.) , Vol. 277, Issue 5327, pp. 818-21, 1997/01 (PubMed)

Wu, Burns, Zhan et al.:
"Molecular cloning and functional analysis of the mouse homologue of the KILLER/DR5 tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) death receptor."
In: Cancer research , Vol. 59, Issue 12, pp. 2770-5, 1999/01 (PubMed)

Hayakawa, Screpanti, Yagita et al.:
"NK cell TRAIL eliminates immature dendritic cells in vivo and limits dendritic cell vaccination efficacy."
In: Journal of immunology (Baltimore, Md. : 1950) , Vol. 172, Issue 1, pp. 123-9, 2003/01 (PubMed)

Sato, Nakaoka, Yamashita et al.:
"TRAIL-transduced dendritic cells protect mice from acute graft-versus-host disease and leukemia relapse."
In: Journal of immunology (Baltimore, Md. : 1950) , Vol. 174, Issue 7, pp. 4025-33, 2005/01 (PubMed)

Takeda, Yamaguchi, Akiba et al.:
"Induction of tumor-specific T cell immunity by anti-DR5 antibody therapy."
In: The Journal of experimental medicine , Vol. 199, Issue 4, pp. 437-48, 2004/01 (PubMed)

External Information:  DR5 Mouse (Murine) antibody

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Google Scholar Find more references for clone MD5-1 on Google Scholar™
EMBL Harvester Search human, mouse or rat genome for antigen DR5 (Bioinformatic Harvester (EMBL))