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产品详细信息： Defensin alpha 1-3 (Human) antibody
Defensin alpha 1-3 (Human) antibody
概述
| 抗原: | Defensin alpha 1-3 |
| 抗体类型: | 单克隆抗体 |
| 应用范围: | Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) |
| 目标物种: | Human |
| 抗体来源: | Mouse |
| 规格: | |
| 价格: | 3.953,49 ¥ (加上运费和增值税, Invoice in EUR) |
| 货号: | ABIN118975 |
| 发货时间: | Ships in 3 to 10 days |
详细信息: Defensin alpha 1-3 (Human) antibody
| 抗原 | Defensin alpha 1-3 |
| 目标物种 | Human |
| 抗体类型 | 单克隆抗体 |
| 状态 | Purified |
| 抗体同种型 | IgG1 »相配的二抗 |
| 克隆 | DEF3 |
| 抗体来源 | Mouse |
| 特异性 | Defensin 1-3 (HNP-1 to HNP-3) in human neutrophils. It is not known whether it reacts with Defensin-4. A side reaction to elastin has been observed in humans. Species crossreactivity is not known. Defensins have been described in rabbits (NP-1 to NP-5, NP3a = Corticostatin), and in mice (cryptodin). DEF-3 recognises a family of cyclic peptides in neutrophils. Four of these peptides are described in humans (HNP-1 to 4), six in rabbits (NP-1 to 5). Synonyms are MCP-1 for NP-1, MCP-2 for NP-2 and corticostatin for NP-3. The function of these peptides apart from their bactericidal, antifungal, and monocyte chemotactic functions is the inhibition of ACTH - induced corticosteroid synthesis. DEF-3 is an important marker for inflammation typing, and for staining mature neutrophils in immunohistochemistry. Defensins are a group of cyclic peptides containing 29-35 amino acids (MW < 3500) which tend to form aggregates. The molecules are protease resistant. The Defensin content of azurophilic granules in neutrophils is approximately 30% of the total protein. Various functions have been described for Defensins. They are antibacterial, antifungal, chemotactic for monocytes, inhibitory for ACTH-induced corticosteroid synthesis and cytotoxic for cells. Defensins are inhibited by glucosaminoglycans (self protection for cells) and high concentrations of Ca2+ ions. |
使用细节: Defensin alpha 1-3 (Human) antibody
| 应用范围 | Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) |
| 应用备注 | Flow cytometry with permeabilized cells (use 5 ul of neat antibody to label 106 cells). Immunohistochemistry on frozen (0.2 ug/ml or 1:5000) Fixation: Acetone and formalin fixed paraffin embedded sections (requires pretreatment by enzyme digestion). ELISA (protocol see below) Other applications not tested. Optimal dilutions of this antibody are dependent on conditions and should be determined by the user. |
| 浓度 | 1.0 mg/ml |
| 缓冲液 | This antibody is supplied as liquid, purified immunoglobulin fraction in PBS buffer pH 7.2 with 0.09% sodium azide as preservative. |
| 贮藏 | Store short term (up to 2 weeks) at 4°C or aliquot and store at -20°C to -70°C, do not freeze working dilutions. Avoid repeated freezing and thawing. Shelf life: one year from despatch. |
| 研究领域 | Immunology |
| 限制 | Research only |
参考文献: Defensin alpha 1-3 (Human) antibody
| 参考文献 |
Schluesener, Meyermann: "Neutrophilic defensins penetrate the blood-brain barrier." In: Journal of neuroscience research , Vol. 42, Issue 5, pp. 718-23, 1996/01 (PubMed) |
