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Application
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ELISA, Western Blotting (WB), Immunoprecipitation (IP)
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Application Notes
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Note This monoclonal antibody reacts specifically with DNP (2,4-dinitrophenol). The antibody reacts with free DNP, DNP conjugated to carriers such as thyroglobulin or BSA, and detects the presence of DNP in proteins and nucleic acids. Although not tested, this antibody is likely functional in RIA, flow cytometry, immunohistochemistry and immunoprecipitation. Recommended Dilutions: This product was assayed against DNP conjugated BSA by ELISA using HRP Goat-anti-Armenian Hamster IgG for detection. A working dilution of 1:2,000 to 1:10,000 is suggested for this assay. For immunoblotting dilute the antibody 1:1000 immediately before use. Researchers should determine optimal titers for other applications.
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Concentration
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1.0 mg/ml (by UV absorbance at 280 nm)
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Purity
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0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2Stabilizer: None Preservative: 0.01% (w/v) Sodium Azide
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Purification
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This product is an IgG fraction antibody purified from ascites or tissue culture supernate by affinity chromatography followed by extensive dialysis against the buffer stated above. Assay by SDS-PAGE resulted in a single protein band. The antibody reacts with DNP but not with carrier protein.
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Storage
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Store vial at -20° C prior to opening. Centrifuge product if not completely clear after standing at room temperature. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of opening.
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Research Area
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Toxins
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Restrictions
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For Research Use only
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