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Application
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Immunofluorescence (IF), Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
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Application Notes
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Western blotting, immunohistochemistry, and ELISASuggested working dilutions: *Western Blot - 1:5,000-1:100,000 (chromogenic)Western Blot - 1:10,000-1:200,000 (ECL)Immunohistochemistry - 1:500-1:5,000 (tissue sections)*The investigator should determine the optimal working dilution for a specific applicationand detection system.
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Conjugate
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HRP
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Concentration
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0.8 mg/ml
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Buffer
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0.5 ml of antibody in 0.01M Sodium Phosphate and 0.25 M NaCl (pH 7.6). Antibodyconcentration is 0.8 mg/ml. Isolated from antisera by immunoaffinity chromatographyusing antigens coupled to agarose beads. Preservative: None (Use of Sodium Azide as a preserv
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Storage
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Store at 4 degrees C. Long-term storage, add 50%ƒc glycerol (ACS grade or better) andstore at –20 degrees C. Avoid repeated freeze-thaw cycles. Protein concentration andbuffer salts will decrease by ½, of the original, after the addition of glycerol.
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Research Area
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Secondary Antibodies
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Restrictions
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For Research Use only
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