BASKET
HELP
Product details for Donkey anti-Goat Goat IgG (H&L) Fab Fragment , Biotin
Donkey anti-Goat Goat IgG (H&L) Fab Fragment , Biotin
Overview
| Antigen: | IgG H&L Fab Fragment |
| Antibody Type: | Polyclonal |
| Conjugate: | Biotin |
| Host: | Donkey |
| Quantity: | |
| Price: | 285,49 $ (Plus shipping costs and VAT, Invoice in EUR) |
| Order number: | ABIN117616 |
| Availability: | Ships in 3 to 10 days |
Additional Information: Donkey anti-Goat Goat IgG (H&L) Fab Fragment , Biotin
| Antigen | IgG H&L Fab Fragment |
| Immunogen | Goat IgG whole molecule |
| Antibody Type | Polyclonal |
| Host | Donkey |
| Specificity | This product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, papain digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin and anti-Donkey Serum. No reaction was observed against anti-Papain or anti-Donkey IgG F(c). |
Application Details: Donkey anti-Goat Goat IgG (H&L) Fab Fragment , Biotin
| Application Notes | Immunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency. This product has been assayed against 1.0 ug of Goat IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:8,000 to 1:36,000 of the reconstitution concentration is suggested for this product. Optimal dilutions of this antibody are dependent on conditions and should be determined by the user. |
| Conjugate | Biotin |
| Concentration | 1.0 mg/ml (by UV absorbance at 280 nm) |
| Buffer | This antibody is supplied as liquid IgG fraction in 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 10 mg/ml Bovine Serum Albumin (BSA) IgG and Protease free as stabilizer and 0.01% (w/v) Sodium Azide as preservative. Label: Biotinamidoca |
| Storage | Store vial at 4° C prior to restoration. Restore with 1.0 ml of deionized water (or equivalent). For extended storage mix product with glycerol to 50% and then aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of restoration. |
| Research Area | Secondary Antibodies |
| Restrictions | Research only |
Publications: Donkey anti-Goat Goat IgG (H&L) Fab Fragment , Biotin
| Publications |
Bayer, Wilchek: "Protein biotinylation." In: Methods in enzymology , Vol. 184, pp. 138-60, 1990/01 (PubMed) |
External Information: Donkey anti-Goat Goat IgG (H&L) Fab Fragment , Biotin
| Google Scholar | Find more references for antigen IgG H&L Fab Fragment on Google Scholar™ |
| EMBL Harvester | Search human, mouse or rat genome for antigen IgG H&L Fab Fragment (Bioinformatic Harvester (EMBL)) |
browse antibodies by
Antigens
Secondary Antibodies
Research areas
Clones
Hosts
Applications
Reactivity
Supplier
Antigens
Secondary Antibodies
Research areas
Clones
Hosts
Applications
Reactivity
Supplier
