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Product details for Donkey anti-Mouse (Murine) Mouse IgG (H&L) F(ab\')2 Fragm...
Donkey anti-Mouse (Murine) Mouse IgG (H&L) F(ab\')2 Fragment multi-species ads.
Overview
| Antigen: | IgG H&L F(ab')2 Fragment |
| Antibody Type: | Polyclonal |
| Conjugate: | PE |
| Host: | Donkey |
| Quantity: | |
| Price: | 448,63 $ (Plus shipping costs and VAT, Invoice in EUR) |
| Order number: | ABIN117869 |
| Availability: | Ships in 3 to 10 days |
Additional Information: Donkey anti-Mouse (Murine) Mouse IgG (H&L) F(ab\')2 Fragment multi-species ads.
| Antigen | IgG H&L F(ab')2 Fragment |
| Immunogen | Mouse IgG whole molecule Purity / Specificity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Phycoerythrin, anti-Donkey Serum, Mouse IgG and Mouse Serum. No reaction was observed against anti-Pepsin, anti-Donkey IgG F(c), Bovine, Chicken, Goat, Guinea Pig, Syrian Hamster, Horse, Human, Rabbit, Rat or Sheep Serum Proteins or other Mouse heavy or light chain proteins. |
| Antibody Type | Polyclonal |
| Host | Donkey |
Application Details: Donkey anti-Mouse (Murine) Mouse IgG (H&L) F(ab\')2 Fragment multi-species ads.
| Application Notes | Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. The maximum amount of reagent required to stain 1 x 106 cells in flow cytometry is approximately 1.0 ug of antibody conjugate. Lesser amounts of reagent may be sufficient for staining. Optimal titers for other applications should be determined by the researcher. As a general guideline dilutions of 1:100 to 1:250 should be suitable for most applications.. |
| Conjugate | PE |
| Concentration | 0.5 mg/ml (determined by absorbance = 82.0 at 565 nm for a 1% solution for only those R-PE molecules to which at least one molecule of active antibody is bound) |
| Buffer | Lyophilized from steril filtered Ig fraction in 0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.6 containing 15 mg/ml BSA (IgG and protease free) as stabilizer and 0.05% (w/v) Sodium Azide, 0.01% (w/v) Thimerisol as preservatives. Conjugated to R- |
| Research Area | Secondary Antibodies |
| Restrictions | Research only |
External Information: Donkey anti-Mouse (Murine) Mouse IgG (H&L) F(ab\')2 Fragment multi-species ads.
| Google Scholar | Find more references for antigen IgG H&L F(ab')2 Fragment on Google Scholar™ |
| EMBL Harvester | Search human, mouse or rat genome for antigen IgG H&L F(ab')2 Fragment (Bioinformatic Harvester (EMBL)) |
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