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Produktdetails für F4 / 80 (Mouse) antibody
F4 / 80 (Mouse) antibody
Übersicht
| Antigen: | F4 / 80 |
| Antikörper-Typ: | Monoklonal |
| Applikation: | Flow Cytometry (FACS), Immunohistochemistry ((Frozen Sections) (IHC) (fro)), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Radioimmunoassay (RIA), Western Blotting (WB) |
| Reaktivität: | Mouse (Murine) |
| Wirt: | Rat |
| Menge: | |
| Preis: | 330,00 € (Zzgl. Versandkosten und MWSt) |
| Bestellnummer: | ABIN145735 |
| Verfügbarkeit: | 7 to 10 days until shipment |
Produktbeschreibung: F4 / 80 (Mouse) antibody
| Antigen | F4 / 80 |
| Immunogen | Thioglycollate stimulated peritoneal macrophages from C57/BL mice |
| Reaktivität | Mouse (Murine) |
| Antikörper-Typ | Monoklonal |
| Format | Liquid |
| Isotyp | IgG2b »Passende Sekundärantikörper |
| Beschreibung | The antibody binds to macrophages from different sites including the peritoneal cavity, lung, spleen and thymus, to blood monocytes and to macrophages derived from bone marrow precursors in culture. The antigen is not expressed by lymphocytes or polymorphonuclear cells. |
| Klon | CI:A3-1 |
| Wirt | Rat |
| Spezifität | This antibody recognises the mouse F4/80 antigen, a 160kD glycoprotein expressed by murine macrophages. |
Anwendungen: F4 / 80 (Mouse) antibody
| Applikation | Flow Cytometry (FACS), Immunohistochemistry ((Frozen Sections) (IHC) (fro)), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Radioimmunoassay (RIA), Western Blotting (WB) |
| Applikationshinweise | Flow Cytometry (Use 10ul of the suggested working dilution to label 10^6 cells)Immunohistology (frozen, paraffin and resin)-requires protein digestion pre-treatment of paraffin sections e.g. trypsinImmunoprecipitationWestern BlottingRadioimmunoassay |
| Konzentration | 1 mg/ml |
| Reinheit | IgG fraction |
| Buffer | 0.01M Na phosphate, 0.25M NaCl, pH7.6, 0.05% Na azide, 0.01% Thimerisol, 15mg/ml BSA |
| Lagerung | Store at 4 C. Aliquot and store at -20 C long-term. |
| Beschränkungen | For research use only |
Publikationen: F4 / 80 (Mouse) antibody
| Publikationen |
Lee, Starkey, Gordon: "Quantitative analysis of total macrophage content in adult mouse tissues. Immunochemical studies with monoclonal antibody F4/80." In: The Journal of experimental medicine , Vol. 161, Issue 3, pp. 475-89, 1985/01 (PubMed) Austyn, Gordon: "F4/80, a monoclonal antibody directed specifically against the mouse macrophage." In: European journal of immunology , Vol. 11, Issue 10, pp. 805-15, 1982/01 (PubMed) Alves-Rosa, Vermeulen, Cabrera et al.: "Macrophage depletion following liposomal-encapsulated clodronate (LIP-CLOD) injection enhances megakaryocytopoietic and thrombopoietic activities in mice." In: British journal of haematology , Vol. 121, Issue 1, pp. 130-8, 2003/01 (PubMed) Beck, Voswinckel, Wagner et al.: "Participation of bone marrow-derived cells in long-term repair processes after experimental stroke." In: Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism , Vol. 23, Issue 6, pp. 709-17, 2003/01 (PubMed) |
Weitere Informationen: F4 / 80 (Mouse) antibody
| Google Scholar | Suchen Sie in Google Scholar™ nach weiteren Artikeln für Klon CI:A3-1 |
| EMBL Harvester | Genom von Mensch, Maus oder Ratte nach Antigen F4 / 80 durchsuchen (Bioinformatic Harvester (EMBL)) |
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