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Product details for F4/80 gen FITC (Mouse (Murine)) antibody
F4/80 gen FITC (Mouse (Murine)) antibody
Overview
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| Antigen: | Macrophages (F4/80 Antigen) |
| Antibody Type: | Monoclonal |
| Application: | Flow Cytometry (FACS) |
| Conjugate: | FITC |
| Reactivity: | Mouse (Murine) |
| Host: | Rat |
| Quantity: | |
| Price: | 360,18 $ (Plus shipping costs and VAT, Invoice in EUR) |
| Order number: | ABIN111853 |
| Availability: | 3 to 10 days until shipment |
Additional Information: F4/80 gen FITC (Mouse (Murine)) antibody
| Antigen | Macrophages (F4/80 Antigen) |
| Immunogen | Thioglycollate stimulated peritoneal macrophages from C57/BL mice |
| Reactivity | Mouse (Murine) |
| Antibody Type | Monoclonal |
| Isotype | IgG2b »Matching secondary antibodies |
| Clone | CI:A3-1 |
| Host | Rat |
| Specificity | ABIN111853 recognises the mouse F4/80 antigen, a 160kD glycoprotein expressed by most murine macrophages. The antibody binds to macrophages from different sites including the peritoneal cavity, lung, spleen and thymus, to blood monocytes and to macrophages derived from bone marrow precursors in culture. The antigen is not expressed by lymphocytes or polymorphonuclear cells. |
Application Details: F4/80 gen FITC (Mouse (Murine)) antibody
| Application | Flow Cytometry (FACS) |
| Application Notes | Flow Cytometry. Other applications not tested. Optimal dilutions of this antibody are dependent on conditions and should be determined by the user. |
| Conjugate | FITC |
| Concentration | 0.1 mg/ml |
| Buffer | This antibody is supplied as liquid, Protein G purified immunoglobulin fraction conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) in PBS Buffer pH 7.4 with 0.09 % Sodium Azide as preservative and 1 % Bovine Serum Albumin. |
| Storage | Store the antibody undiluted at 4-8°C for one month or at –20°C for longer. Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light. Shelf life: one year from despatch. |
| Research Area | Cell/Tissue Markers, Inflammation |
| Restrictions | For Research Use only |
Publications: F4/80 gen FITC (Mouse (Murine)) antibody
| Publications |
Austyn, Gordon: "F4/80, a monoclonal antibody directed specifically against the mouse macrophage." In: European journal of immunology , Vol. 11, Issue 10, pp. 805-15, 1982/01 (PubMed) Gordon, Lawson, Rabinowitz et al.: "Antigen markers of macrophage differentiation in murine tissues." In: Current topics in microbiology and immunology , Vol. 181, pp. 1-37, 1992/01 (PubMed) Hume, Perry, Gordon: "The mononuclear phagocyte system of the mouse defined by immunohistochemical localisation of antigen F4/80: macrophages associated with epithelia." In: The Anatomical record , Vol. 210, Issue 3, pp. 503-12, 1985/01 (PubMed) Lee, Starkey, Gordon: "Quantitative analysis of total macrophage content in adult mouse tissues. Immunochemical studies with monoclonal antibody F4/80." In: The Journal of experimental medicine , Vol. 161, Issue 3, pp. 475-89, 1985/01 (PubMed) |
Images: F4/80 gen FITC (Mouse (Murine)) antibody
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Staining of mouse peritoneal macrophages with RAT ANTI MOUSE F4/80 ANTIGEN:FITC (ABIN111853). |
External Information: F4/80 gen FITC (Mouse (Murine)) antibody
| Google Scholar | Find more references for clone CI:A3-1 on Google Scholar™ |
| EMBL Harvester | Search human, mouse or rat genome for antigen Macrophages (F4/80 Antigen) (Bioinformatic Harvester (EMBL)) |
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