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Product details for  FOXP3 Human antibody
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FOXP3 Human antibody

Overview

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Antigen: FOXP3
Antibody Type: Monoclonal
Application: Intracellular Flow Cytometry (ICFC)
Conjugate: Pacific Blue
Reactivity: Human
Host: Mouse
Quantity: 25 tests (Product variants)
Price: 266,53 $   (Plus shipping costs and VAT, Invoice in EUR)
Order number: ABIN159142
Availability: Ships within 7 to 10 days
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Additional Information:  FOXP3 Human antibody

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Antigen FOXP3
Immunogen Full-length FOXP3 protein
Reactivity Human
Antibody Type Monoclonal
Isotype Mouse IgG1, kappa »Matching secondary antibodies
Description FOXP3 is a 50-55 kD transcription factor, also known as Forkhead box protein P3, Scurfin, JM2, or IPEX. It is proposed to be a master regulatory gene and more specific marker of T regulatory cells than most cell surface markers (such as CD4 and CD25). Transduced expression of FOXP3 in CD4/CD25 cells has been shown to induce GITR, CD103, and CTLA4 and impart a T regulatory cell phenotype. FOXP3 is mutated in X-linked autoimmunity-allergic dysregulation syndrome (XLAAD or IPEX) in humans and in scurfy mice. Overexpression of FOXP3 has been shown to lead to a hypoactive immune state suggesting that this transcriptional factor is a central regulator of T cell activity. In human, unlike in mouse, two isoforms of FOXP3 have been reported: one (FOXP3) corresponding to the canonical full-length sequence, the other (FOXP3 delta2) lacking exon 2. The 206D antibody recognizes human FOXP3 epitope in the region of amino acids 105-235. Distribution: Nuclear, expressed in T regulatory cells Function: Transcription factor proposed to be a master regulatory gene in T regulatory cell development and a critical factor for immune homeostasis Regulation: FOXP3 is present at high levels in T regulatory cell can also be induced by T cell activation Structure: Forkhead/winged-helix transcription factor family, approximately 50 kD, contains zinc finger and forkhead domains
Clone 206D
Host Mouse
Specificity Interacts with DNA.

Application Details:  FOXP3 Human antibody

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Application Intracellular Flow Cytometry (ICFC)
Conjugate Pacific Blue
Purification The antibody was purified by affinity chromatography, and conjugated with Pacific Blue(TM) under optimal conditions. The solution is free of unconjugated Pacific Blue(TM) .
Buffer Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Storage The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Restrictions For Research Use only

Publications:  FOXP3 Human antibody

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Publications Allan, Passerini, Bacchetta et al.:
"The role of 2 FOXP3 isoforms in the generation of human CD4+ Tregs."
In: The Journal of clinical investigation , Vol. 115, Issue 11, pp. 3276-84, 2005/01 (PubMed)

Bennett, Christie, Ramsdell et al.:
"The immune dysregulation, polyendocrinopathy, enteropathy, X-linked syndrome (IPEX) is caused by mutations of FOXP3."
In: Nature genetics , Vol. 27, Issue 1, pp. 20-1, 2001/01 (PubMed)

Ferguson, Blanton, Saulsbury et al.:
"Manifestations and linkage analysis in X-linked autoimmunity-immunodeficiency syndrome."
In: American journal of medical genetics , Vol. 90, Issue 5, pp. 390-7, 2000/01 (PubMed)

Fontenot, Gavin, Rudensky:
"Foxp3 programs the development and function of CD4+CD25+ regulatory T cells."
In: Nature immunology , Vol. 4, Issue 4, pp. 330-6, 2003/01 (PubMed)

Hori, Nomura, Sakaguchi:
"Control of regulatory T cell development by the transcription factor Foxp3."
In: Science (New York, N.Y.) , Vol. 299, Issue 5609, pp. 1057-61, 2003/01 (PubMed)

Images:  FOXP3 Human antibody

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CD4+CD25+ (top) or CD4+CD25- (bottom) human peripheral blood lymphocytes intracellular stained with 206D Pacific Blue(TM) (red line) or mouse IgG1, kappa isotype control Pacific Blue(TM) (blue line)

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External Information:  FOXP3 Human antibody

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Google Scholar Find more references for clone 206D on Google Scholar™
EMBL Harvester Search human, mouse or rat genome for antigen FOXP3 (Bioinformatic Harvester (EMBL))